RNAi沉默STAT3基因对结肠癌HCT116细胞侵袭的调控  

Regulation of STAT3 siRNA on Invasion of Colon Cancer Cell Line HCT116

作　　者：范钰[1] 张尤历[1] 张字川 吴莺[1] 许则丰[2] 万毅刚[3] 

机构地区：[1]江苏大学附属医院消化科,江苏镇江212001 [2]浙江大学肿瘤研究所,浙江杭州310009 [3]南京大学医学院附属鼓楼医院中医科,江苏南京210008

出　　处：《中国临床医学》2007年第4期512-514,共3页Chinese Journal of Clinical Medicine

基　　金：镇江市科技计划项目(SH2006019)

摘　　要：目的:观察RNA干扰沉默STAT3基因对结肠癌细胞侵袭的影响,并探讨其可能机制。方法:应用STAT3小干扰RNA(small interfering RNA,siRNA)转染处理人结肠癌细胞系HCT116后,采用RT-PCR检测STAT3 mRNA表达,软琼脂集落培养试验检测锚着不依赖性增殖,Boyden小室模型试验检测癌细胞的侵袭性,琼脂糖凝胶电泳和TUNEL检测癌细胞失巢凋亡。结果:STAT3 siRNA可有效抑制结肠癌细胞集落生长和侵袭能力,且与浓度相关。琼脂糖凝胶电泳和TUNEL结果显示,STAT3 siRNA转染可诱导结肠癌细胞失巢凋亡,且与浓度相关。结论:STAT3 siRNA可抑制结肠癌细胞的恶性侵袭,其机制与诱导失巢凋亡有关。Objective： To study the effects of STAT3 small interfering RNA（siRNA）on invasion of colon cancer cell line HCT116. Methods：After colon cancer HCT116 cell were transfected by STAT3 siRNA, we studied the expression of STAT3 mRNA using RT-PCR, anchorage-independent growth using clon formation in soft agar,and invasion using boyden chamber model,anoikis using and DNA fragmentation assay and terminal deoxynucleotidyltransferase-mediated dUTP nick-end labeling （TUNEL）. Results： STAT3 siRNA can inhibit anchorage-independent growth and invasion ability in a dose-dependent manner. STAT 3 can reduce the resistance to anoikis of HCT116 cells in a dose- and time-dependent manner. Conclusion： STAT 3 siRNA can inhibit anchorage-independent growth and invasion ability of colon cancer cell through inducing anoikis.

关 键 词：结肠肿瘤 信号转导子和转录激活子3 RNA干扰 小干扰RNA 侵袭 失巢凋亡 

分 类 号：R735.35[医药卫生—肿瘤]