BCSC-1基因异位表达致鼻咽癌细胞CNE-2L2黏附性增强及细胞周期阻滞  

Ectopic Expression of BCSC-1 Gene Results in Enhancement of Adhesion and Cell Cycling Blockade of Nasopharyngeal Carcinoma CNE-2L2 Cell

作　　者：陈双玲[1] 周异群[1] 田云[1] 鞠吉雨[1] 刘音[1] 朱立平[1] 

机构地区：[1]中国医学科学院北京协和医学院基础医学院免疫学系,北京100005

出　　处：《中国医学科学院学报》2007年第4期533-537,共5页Acta Academiae Medicinae Sinicae

基　　金：国家重点基础研究发展计划项目(973计划)(2001CB510004);国家高技术研究发展计划项目(863项目)(2002BA711A03)~~

摘　　要：目的探讨BCSC-1基因异位表达导致人鼻咽癌细胞CNE-2L2恶性行为减弱的机制。方法采用碘化丙啶染色DNA,流式细胞法检测细胞周期;Hoechest33258染色分析细胞分裂情况;细胞聚集实验检测细胞的黏附性;Western印迹分别检测E-钙黏蛋白、α-catenin和p53的表达。结果细胞周期分析显示异位表达BCSC-1的CNE-2L2细胞、野生型CNE-2L2细胞和转染空载体的CNE-2L2细胞分别有55.1%、43.4%和39.4%处于G0/G1期,分别有25.2%、28.7%和30.9%处于S期,分别有19.7%、27.9%和29.7%处于G2/M期。野生型CNE-2L2细胞和转染空载体的CNE-2L2细胞有较多细胞处于有丝分裂相,异位表达BCSC-1的CNE-2L2细胞几乎见不到有丝分裂相。异位表达BCSC-1的CNE-2L2细胞的黏附性和E-钙黏蛋白、α-catenin及p53的表达水平均高于野生型CNE-2L2细胞和转染空载体的CNE-2L2细胞。结论异位表达BCSC-1的CNE-2L2细胞的黏附性增强可能与E-钙黏蛋白和α-catenin表达增强相关;异位表达BCSC-1的CNE-2L2细胞阻滞于G1期可能与p53表达增加相关。这些变化可能在细胞恶性行为减弱中发挥作用。Objective To study mechanisms of reduction of the malignant activities of human nasopharyngeal carcinoma cell CNE-2L2 induced by ectopic expression of BCSC-1 gene. Methods DNA was stained with propidium iodide and assayed upon a flow cytometer. Chromosomes were stained with Hoechest 33258. Adhesion of CNE-2L2 cells was detected by cell aggregation test. Protein expression on CNE-2L2 cells was examined by Western blot. Results Cell cycle analysis showed that the percentage of CNE-2L2 cells was 55. 1%, 43.4%, and 39.4% in G0/G1 phase, 25.2%, 28.7%, and 30.9% in S phase, and 19.7%, 27.9% , and 29. 7% in G2/M phase for the cell with ectopic expression of BCSC-1 gene, wild type cell （ W cells）, and the cell transduced with the mock （ M cell）. Many mitotic cells were found in W cells and M cells. In contrast, almost no mitotic cell was observed in the cells with ectopic expression of BCSC-1 gene.Ectopic BCSC-1 expression resulted in cell aggregation, enhanced expression of E-cadherin, α-catenin, and p53. Conclusions Ectopic BCSC-1 expression causes enhancement of adhesion of CNE-2L2 cells associated with enhanced expression of E-cadherin and α-catenin, arrest of cell in G1 phase, which may be associated with enhanced expression of p53. These alteration may play a role in the reduction of malignant activities of the cells with ectopic expression of BCSC-1 gene.

关 键 词：人鼻咽癌细胞CNE-2L2 BCSC-1基因 黏附性 细胞周期 E-钙黏蛋白 Α-CATENIN p53 

分 类 号：R739.63[医药卫生—肿瘤]