大鼠角膜微囊袋植入微粒体诱导新生血管模型的研究  

Study on the Rat Models of Corneal Neovascularization Induced by Micropocket Assay

作　　者：赵伟[1] 凌士奇[1] 刘祖国[2] 

机构地区：[1]广州市儿童医院,广东广州510120 [2]厦门眼科中心,福建厦门361003

出　　处：《实用医技杂志》2007年第25期3425-3427,共3页Journal of Practical Medical Techniques

摘　　要：目的:探讨大鼠角膜微囊袋植入微粒体诱导新生血管模型的制作技巧和特点。方法:9只SD大鼠手术显微镜下做角膜基质微囊袋并植入含有VEGF的缓释微粒体。术后1 d、3 d、5 d、7 d采用裂隙灯显微镜观察角膜新生血管的生长情况。结果:术后1 d大鼠角膜缘血管网充血扩张,无新生血管生长;3 d可见毛刷状新生血管自角膜缘伸入角膜,面积为(2.23±0.59)mm^2;5 d新生血管垂直于角膜缘向微粒体方向束状延伸,面积为(6.81±1.35)mm^2;7 d新生血管继续向前生长,部分新生血管顶端互相吻合成袢状,面积为(8.92±1.79)mm^2。所有术眼均未见前房积血、角膜溃疡、微粒体脱出等并发症。结论:大鼠角膜微囊袋植入微粒体诱导的新生血管生长稳定、无并发症、适用于定量研究。Objective To explore the skills and characteristics of corneal neovascular model in rat induced by micropocket assay. Methods Nine eyes of nine Sprague-Dawley rats were studied. Pellets made of VEGF, poly-2-hydroxylethylmethacrylate and sucralfate were implanted into the corneal stroma no closer than 1 mm from the limbus. Biomicroscopic features of corneal neovascular were observed on 1, 3, 5, 7 day after the implantation. Results The First day after operation, the limbal vessels were dilated, with no angiogenesis appeared. The Third day, angiogenesis began to invade peri-cornea with a brush shape, the area of CNV was （2.23 ±0.59）mm^2. The Fifth day, new vessels reached the lower margin of pellet densely, the area of CNV was （6.81± 1.35） mm^2. The Seventh day, new vessels continued to elongate, parts of them extended as loops toward the pellet, the area of CNV was （8.92 ± 1.79）mm^2. Neither hyphema or other complications was occurred. Conclusion Corneal neovascular induced by micropocket assay in rat grows steadily, with no complication, and is suitable for quantitative researches.

关 键 词：角膜新生血管 微囊袋法 微粒体 

分 类 号：R-332[医药卫生]