太原地区妇女儿童淋球菌感染调查及基因快速诊断  

Using Polymerase Chain Reaction for the Detection of Gonorrhoea in Women and Children in Taiyuan

作　　者：李连青[1] 朱庆义[1] 贺润花[1] 刘建萍[1] 赵瑞珍[1] 

机构地区：[1]山西省妇幼保健院儿童医院分子微生物研究室,太原030013

出　　处：《中华流行病学杂志》1997年第2期98-101,共4页Chinese Journal of Epidemiology

摘　　要：改进并建立快速检测淋球菌基因（NG－DNA）的聚合酶链反应（PCR），同时用涂片染色筛查和培养法作鉴定比较，对太原地区1986～1995年妇女儿童淋球菌感染状况作调查分析，对9204例妇女儿童生殖道感染者作直接涂片染色检菌，阳性率为26.5％，其中1220例同时用培养与PCR法检测，阳性率为12．4％和21．0％。用PCR直接检测临床标本NG－DNA，具有简便、快速、敏感、特异之优点。A polymerase chain reaction (PCR) method was established for the detection of gonococcal gene in samples obtained from women and children with lower genital tract infection. The primers were used for PCR amplification derived from sequence for DNA representing part of the open reading frame (ORF) for the chromosome of N.gonorrhoea.In this study,a total number of 9204specimens were obtained from women and children with lower genital tract infection from 1986 to 1995in Taiyuan. The positive rate of N. gonorrhoea for Gram's stain of smears was 26. 5 % while the rates for culture and PCR were 12. 4% and 21.0%, respectively. The results showed that PCR test was simple,rapid sensitive and specific for the detection of gonococcal gene in clinical specimens.

关 键 词：聚合酶链反应 诊断 淋病 基因诊断 妇女 儿童 

分 类 号：R759.220.4[医药卫生—皮肤病学与性病学] R759.230.4[医药卫生—临床医学]