小鼠实验性腹膜炎回肠组织损伤性变化以及苯妥英钠干预的影响  

Damage of Ileum in Experimental Peritonitis in mice and Effect of Phenytoin Sodium Intervention

作　　者：李海峰[1] 李英[2] 程建青[2] 范红斌[2] 

机构地区：[1]河北北方学院电镜室,075000 [2]江南大学医学院基础医学部

出　　处：《胃肠病学》2007年第8期481-483,共3页Chinese Journal of Gastroenterology

摘　　要：背景:细菌和毒素可通过有或无破损的肠黏膜易位诱发全身炎症反应综合征(SIRS),肠道既可能是SIRS的开始器官,又是SIRS的靶器官。目的:探讨实验性腹膜炎回肠组织损伤性变化以及苯妥英钠干预的影响。方法:30只小鼠随机分为实验组(12、24和48 h)、治疗组和对照组,每组6只。实验组腹腔注射2%冰醋酸0.1 ml,12、24和48 h后分别取材;治疗组腹腔注射冰醋酸后于2和6 h分别腹腔注射1%苯妥英钠0.2 ml,造模后24 h取材;对照组腹腔注射等量0.9%NaCl溶液。观察实验组回肠组织的病理学变化,测定乳酸脱氢酶(LDH)、肌酸激酶(CK)活性和丙二醛(MDA)、还原型谷胱甘肽(GSH)含量。结果:实验组回肠组织发生病理学改变。文验24 h和48 h组的LDH和CK活性较对照组显著降低;实验12 h、24 h和48 h组的MDA含量较对照组显著升高,而GSH含量显著降低。除CK活性外,治疗组的其余指标均较实验24 h组有所改善。结论:实验性腹膜炎回肠组织能量代谢障碍、氧自由基增高参与了组织损伤,早期应用苯妥英钠治疗具有抗自由基损伤和改善能量代谢的作用。Background： Systemic inflammatory response syndrome （SIRS） may be induced by translocation of bacteria and toxin through damaged or undamaged intestinal mucosa. Intestine may be the initiating target as well as the target organ of SIRS. Aims： To investigate the damage of ileum in experimental peritonitis in mice and the effect of phenytoin sodium intervention. Methods： 30 mice were randomly divided into experimental groups （12, 24 and 48 h）, treatment group and control group, 6 mice in each group. The experimental groups were injected intraperitoneally with 0.1 ml 2% glacial acetic acid and killed at 12, 24 and 48 h, respectively. The treatment group was injected intraperitoneally with 0.2 ml 1% phenytoin sodium 2 and 6 h after the injection of glacial acetic acid, and ileum specimens were obtained at 24 h. The control group was injected intraperitoneally with equal amount of 0.9% NaCl. Pathologic changes in ileum in experimental groups were observed, the activities of lactate dehydrogenase （LDH）, creatine kinase （CK） and the levels of malondialdehyde （MDA）, reduced glutathione （GSH） were measured. Results： Intestinal mucosal damages were observed in experimental groups. The activities of LDH, CK in 24 h and 48 h experimental groups decreased significantly than those in control group. The levels of MDA in the three experimental groups increased significantly and levels of GSH decreased significantly. Except the activity of CK, all the other parameters in treatment group improved significantly when compared with those in 24 h experimental group. Conclusions： The damage of ileum in experimental peritonitis in mice is related to the disordered energy metabolism and increasing of oxygen free radicals. Phenytoin sodium has significant protective effect on injured ileum in experimental peritonitis via its anti-oxygen free radicals and improving energy metabolism.

关 键 词：小鼠 腹膜炎 回肠 苯妥英 

分 类 号：R572.2[医药卫生—消化系统]