AML患者外周血中TCR Vβ亚家族naive T细胞的检测  

Analysis of TCR Vβ subfamily naive T cells from peripheral blood of patients with AML

作　　者：尹青松[1] 李扬秋[1] 陈少华[1] 杨力建[1] 

机构地区：[1]暨南大学医学院血液病研究所,广州510632

出　　处：《免疫学杂志》2007年第5期537-540,共4页Immunological Journal

基　　金：国家自然科学基金(30270579);广东省自然科学基金重点项目(23001);广东省教育厅"千百十工程"优秀人才培养基金项目(Q校02022);血液病学国务院侨办重点学科基金资助项目

摘　　要：目的检测急性髓性白血病(AML)患者外周血中TCR23个Vβ亚家族的T细胞受体删除DNA环(sjTRECs)的存在特点,从而间接了解AML患者相应Vβ亚家族naiveT细胞的近期胸腺输出情况。方法利用半巢式PCR分别扩增32例AML患者每5×104和1×104个外周血单个核细胞(PBMCs)中的23个Vβ亚家族sjTRECs,10例正常人外周血作为对照。结果在5×104和1×104两种PBMCs水平,AML患者检出sjTRECs的Vβ亚家族数分别为(5.09±3.28)和(2.59±2.06)个,与正常人的(13.70±2.67)和(5.50±2.07)个相比,两组的差异均有统计学意义(两组均为P=0.000)。AML患者5×104个PBMCs中约可检测到5/23(20%)个Vβ亚家族sjTRECs,并且有13个Vβ亚家族sjTRECs的检出率明显低于正常人水平,其中Vβ10-和Vβ14-Dβ1sjTRECs的检出率最低,而Vβ21最高。不同FAB分型AML患者间的检出差别未显示出统计学意义。32例AML患者检出sjTRECs的Vβ亚家族数量差别较大(1～15个),年龄与检出的亚家族数量呈负相关,<30岁者的检出量高于≥30岁者,尤其在5×104个PBMCs水平差异有统计学意义(r=-0.481,P=0.005)。结论AML患者胸腺近期输出的23个Vβ亚家族naiveT细胞存在不同程度的缺失或水平降低,表明AML患者存在严重的细胞免疫功能缺陷以及T细胞谱系重建的能力和潜能严重受损。Objective To detect the signal joint T-cell receptor excision DNA circles （ sjTRECs） of TCR23 Vβsubfamiliesinmononudear cells of patients with acute myelogenous leukemia （AML）, and to evaluate the recent thymie emigrants of corresponding Vβ subfamily naive T cells of AML patients. Methods sjTRECs of TCR 23 Vβ subfamilies were amplified in genomic DNA from the 5×10^4 and 1×10^4 PBMCs of 32 cases of patients with AML by using semi-nest PCR. Ten normal individuals served as controls. Results The number of detectable Vβ subfamily sjTRECs were （5.09± 3.28） and （2.59 ±2.06） in 5 ×10^4 and 1 ×10^4 PBMCs from AML patients, respectively, which were significant differences （beth P = 0.000） from （13.70 ±2.67） and （5.50 ±2.07） of normal individuals. About 22% （5/23） Vβ-Dβ1 sjTRECs were detected in 5×10^4 PBMCs from AML patients, and the frequencies in 13 Vβ subfamily sjTRECs were significantly lower than those from normal individuals, among which the lowest were Vβ10 and Vβ14-Dβ1 sjTRECs, the most was Vβ21. But the difference was not significant within the different FAB subtypes of AML patients. 1 - 15 Vβ subfamily sjTRECs could be detected from 32 cases of AML patients. There was negative correlation between age and the number of detectable Vβ subfamily sjTRECs in patients with AML： patients who were 〈 30 years tended to be higher number of detectable Vβ subfamily sjTRECs than those≥ 30 years, and which became significant at 5 ×10^4PBMCs level （ r = - 0.481, P = 0.005）. Conclsion The recent thymic emigrants of 23 Vβ subfamily naive T cells are absent or lower level among patients with AML, which suggests that AML patients has severe cellular immtmodeficiency and dramatically low capacity and potential of long term TCR Vβ repertoire reconstitution.

关 键 词：急性髓性白血病 NAIVE T细胞 TRECS 胸腺近期输出功能 

分 类 号：R733.71[医药卫生—肿瘤]