中药成分CDP逆转高甲基化状态的GSTP1基因启动子低转录活性的研究  

Reactivation of Hypermethylated GSTP1 Promoter Activity in MCF-7 Cells by Treatment with a Component of Natural Drug,CDP

作　　者：张军哲[1] 王锦红[1] 覃扬[1] 傅强[1] 

机构地区：[1]四川大学华西基础医学与法医学院生物化学与分子生物学教研室,成都610041

出　　处：《四川大学学报（医学版）》2007年第5期761-765,共5页Journal of Sichuan University(Medical Sciences)

摘　　要：目的研究中药成分CDP逆转高甲基化状态的GSTP1基因启动子低转录活性的作用。方法构建含完全甲基化GSTP1启动子区的重组质粒pGL3-GSTP1prom并转染MCF-7细胞,分别用不同剂量的CDP和5-aza-C作用细胞,检测表达的萤光素酶活性。观察GSTP1启动子转录活性的变化。结果GSTP1启动子区被完全甲基化后转录活性显著降低;CDP能够逆转高甲基化状态下GSTP1基因启动子区的低转录活性且呈剂量依赖关系。结论中药成分CDP能够逆转高甲基化状态的GSTP1基因启动子区的低转录活性。Objective To investigate the reactivation of hypermethylated GSTP1（glutathione S-transferase P1） promoter activity by a component of natural drug,CDP.Methods The recombinant plasmid of pGL3-GSTP1 prom containing hypermethylated GSTP1 gene promoter was constructed and then used to transiently transfect the human breast cancer cell line MCF-7 cell.After treatment with CDP and 5-aza-C,The luciferase activity in cell lysates were assayed.Results Low promoter activities were found in hypermethylated GSTP1 promoter.The promoter activities were reactivated and in a CDP dose-dependent mode.Conclusion CDP has the ability to reactivate the hypermethylated GSTP1 gene promoter activity.

关 键 词：CDP 高甲基化GSTP1启动子 MCF-7 转染 萤光素酶报告基因 

分 类 号：R73-36[医药卫生—肿瘤]