角质细胞生长因子及其受体在CaSki细胞中的表达及其作用  

Expression and Action of Keratinocyte Growth Factor,Keratinocyte Growth Factor Receptor on CaSki Cell

作　　者：陆琳[1] 王东[2] 胡丽娜[1] 陈斌[2] 贾海军[1] 李金科[1] 王登凤[1] 

机构地区：[1]四川大学华西第二医院妇产科 [2]四川绵阳市中心医院

出　　处：《四川大学学报（医学版）》2007年第5期836-838,共3页Journal of Sichuan University(Medical Sciences)

摘　　要：目的确定角质细胞生长因子(KGF)及受体(KGFR)在CaSki细胞中的表达及KGF对CaSki细胞增殖、迁移的影响。方法取对数增长期的CaSki细胞,ELISA和Western blot确定KGF及KGFR在CaSki细胞中的表达;3H-TdR掺入试验测定KGF、KGF抗体对CaSki细胞增殖的影响;Millicell测定KGF、KGF抗体对CaSki细胞迁移的影响。结果1KGF、KGFR在CaSki细胞中有表达;2人重组KGF因子对CaSki细胞的增殖、迁移有促进作用(P<0.05);3用KGF抗体中和CaSki细胞自分泌的KGF后,CaSki细胞的增殖、迁移减弱(P<0.05)。结论KGF和KGFR在CaSki细胞中有表达;人重组KGF及CaSki细胞自分泌KGF对CaSki细胞的增殖、迁移均有促进作用。Objective To investigate the expressions of keratinocyte growth factor（KGF）,keratinocyte growth factor receptor（KGFR） in CaSki cell and action of keratinocyte growth factor on proliferation,migration of the CaSki cell.Methods ELISA and Western blot methods were used to determine the protein expressions of the KGF and KGFR in CaSki cell respectively：3H-Thymidine incorporation method was used to determine the effect of recombinant human KGF and anti-KGF on CaSki cell proliferation;Millicell-PCF was used to determine the effect of recombinant human KGF,anti-KGF on CaSki cell migration.Results Both KGF and KGFR were expressing in the CaSki cell;Recombinant human KGF resulted in a increase in the proliferation and migration of CaSki cells;The proliferation and migration of CaSki cell became weak due to autocrine KGF neutralized by KGF antibodies（P〈0.05）.Conclusion Current results demonstrate that KGF and KGFR express in CaSki cell;Both autocrine and recombinant human KGF have the effect on CaSki cell proliferation and migration.

关 键 词：CASKI KGF KGFR 增殖 迁移 

分 类 号：R737.33[医药卫生—肿瘤]