缓激肽持续作用对胶质瘤细胞内钙离子浓度的影响  

Effect of Continuous Stimulation of Bradykinin on Intracellular Ca^(2+) Concentration in Glioma Cells

作　　者：王萍[1] 付伟[1] 薛一雪[1] 

机构地区：[1]中国医科大学神经生物学教研室,辽宁沈阳110001

出　　处：《解剖科学进展》2007年第3期193-195,199,共4页Progress of Anatomical Sciences

基　　金：国家自然科学基金(30400145;30600060;30570650);辽宁省自然科学基金(20052102);博士学科点专项科研基金(20050159005)

摘　　要：目的探讨缓激肽持续作用引起的胶质瘤细胞内钙离子浓度变化([Ca2+]i)及机制。方法培养大鼠C6胶质瘤细胞,采用[Ca2+]i测定和免疫荧光细胞化学鉴定的方法,观察持续给予缓激肽后胶质瘤细胞内[Ca2+]i的变化和缓激肽B2受体在细胞内的分布。结果在第一次给予缓激肽时,引起[Ca2+]i的峰值最大,随着给予缓激肽次数的增加,缓激肽诱导的[Ca2+]i荧光强度峰值逐渐下降,在第5次加入缓激肽时,[Ca2+]i荧光强度峰值已无显著变化。在此过程中,缓激肽B2受体发生内化,并与胞浆中的质膜微囊蛋白caveolin-1结合。结论持续给予缓激肽可引起胶质瘤细胞内[Ca2+]i浓度规律变化,caveolin-1可能参与此过程。Objective To study the [Ca^2＋]i change and mechanism induced by continuous stimulation of bradykinin in glioma cells. Methods [Ca^2＋]i assay and immunofluorescence staining were used to observe the [Ca^2＋]i change and the location of bradykinin B2 receptors after the continuous treatment with bradykinin in cultured rat C6 glioma cells. Results With the first stimulation of bradykinin, [Ca^2＋]i fluorescence intensity ascends to peak and descends gradually as the continuous stimulation of bradykinin, and with no significant change of [Ca^2＋]i fluorescence intensity at the fifth stimulation. During this process, combination of bradykinin B2 receptors with caveolin-1 was observed in cytoplasma. Conclusions Continuous administration of bradykinin can cause the regular change of [Ca^2＋]i concentration in glioma, in which caveolin-1 may be involved.

关 键 词：缓激肽 B2受体 [CA^2+]I 内化 胶质瘤细胞 

分 类 号：Q189[生物学—神经生物学]