修复抑制剂对不同敏感性作物超氧化物歧化酶、过氧化氢酶和过氧化物酶活性的影响  

INFLUENCE OF RADIATION DAMAGE REPAIR INHIBITOR ON SUPEROXIDE DISMUTASE (SOD), CATALASE (CAT) AND PEROXIDASE (POD) IN DIFFERENT SENSITIVE CROPS

作　　者：宋道军[1] 徐登益[1] 万兆良[1] 何首林[1] 

机构地区：[1]西南农业大学中心室

出　　处：《核农学报》1997年第2期93-96,共4页Journal of Nuclear Agricultural Sciences

摘　　要：辐射损伤修复抑制剂咖啡因（Ｃａｆ）和Ｎａ２ＥＤＴＡ（ＥＤＴＡ）后处理对不同辐射敏感性作物大豆和油菜幼苗超氧化物歧化酶（ＳＯＤ）、过氧化氢酶（ＣＡＴ）和过氧化物酶（ＰＯＤ）活性的影响呈现为较一致的变化规律。两种作物幼苗下胚轴内，ＳＯＤ酶活性均随着辐照剂量增大而增大，出现峰值后又陡然降低。大豆幼苗ＣＡＴ酶活性与ＳＯＤ酶有大致相同的变化规律，而油菜ＣＡＴ酶活性在随辐照剂量变化过程中，在３００～１０００Ｇｙ范围内波动较小。ＰＯＤ酶活性则均是随着辐照剂量的增大而增大。除个别处理、个别剂量外，Ｃａｆ、ＥＤＴＡ后处理与水处理（对照）相比，３种酶的活性有不同程度的提高，说明Ｃａｆ、ＥＤＴＡ在抑制遗传物质ＤＮＡ损伤修复的同时，减轻了因辐射产生的自由基造成的毒害。各处理中，抗辐射的油菜幼苗下胚轴中ＳＯＤ、ＣＡＴ和ＰＯＤ酶活性都高于对辐射敏感的大豆。The activities of superoxide dismutase (SOD),catalase (CAT) and peroxidase (POD) were affected remarkably by 60 Co γ ray irradiation and radiation damage repair inhibitor (Caf, EDTA). SOD, CAT and POD activities showed the similiar change pattern in both soybean (sensitive to radiation) and Brassica napus L. (resistant to radiation) seedlings in all treatments. After reaching the maximum value, SOD activity decreased with the increase of doses. CAT activity had the same change pattern as that of SOD in soybean, while with Brassica napus L., CAT activity remained relatively steady from 300 Gy to 1000Gy. And POD activity increased with the increase of doses. Compared with H 2O treatments, Caf, EDAT post treatments obviously enhanced SOD, CAT and POD activites. With all the treatments, the three enzyme activities were higher in Brassica napus L. than those in soybean seedlings.

关 键 词：咖啡因 作物 过氧化氢酶 辐射育种 

分 类 号：S335.21[农业科学—作物遗传育种] S124.1[农业科学—农艺学]