高压氧干预后局灶性脑缺血再灌注损伤模型大鼠c-fos的表达(英文)  

c-fos expression in rats with focal cerebral ischemia/reperfusion injury following hyperbaric oxygen intervention

作　　者：曹义战[1] 晋兴 查清[3] 王伯良[1] 仲月霞[1] 付国强[1] 何保健[1] 

机构地区：[1]解放军第四军医大学唐都医院急诊科,陕西省西安市710038 [2]西安高新医院检验科,陕西省西安市710075 [3]解放军空军总医院,北京市100036

出　　处：《中国组织工程研究与临床康复》2007年第35期7106-7109,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：解放军第四军医大学唐都医院科研课题资助资金(2001486)~~

摘　　要：背景:高压氧可增加氧弥散能力,从而改善脑水肿和脑组织缺氧,促进病灶区脑细胞的生理功能恢复、侧支循环的建立和脑细胞再生修复。目的:观察高压氧对大鼠急性局灶性脑缺血再灌注损伤后不同时间点c-fos癌基因表达的影响。设计:随机分组动物实验。单位:解放军第四军医大学唐都医院急诊科、西安高新医院检验中心、解放军空军总医院、解放军第四军医大学航空航天医学系高压氧治疗中心。材料:实验于2002-04在解放军第四军医大学航空航天医学系高压氧治疗中心完成,选用65只生后2个月健康雄性SD大鼠。方法:随机摸球法将大鼠分为4组,模型组(n=20):参照Koizumi等设计的方法,制备大鼠大脑中动脉缺血模型;正常对照组(n=5):手术方式同模型组,但不阻断动脉血流;纯氧治疗组(n=20):手术过程同模型组,动物缺血1h后抽出栓子,分别在插入栓子后2,9,21,45,69h将动物置于高压舱内,常压下吸100%纯氧。高压氧治疗组(n=20):手术过程同模型组,动物缺血1h后抽出栓子,分别在插入栓子后2,9,21,45,69h将动物置于高压氧舱内,0.25MPa吸纯氧1h。主要观察指标:利用免疫组织化学和病理组织学法观察各组大鼠脑缺血再灌注5,12,24,72h脑皮质、视前区与纹状体中性白细胞浸润及c-fos癌基因蛋白及阳性细胞表达的变化;计算脑缺血再灌注72h皮质、纹状体内侧区与视前区神经元坏死程度、大鼠左侧半球脑血管渗漏面积。结果:纳入大鼠65只均进入结果分析。①视前区c-fos阳性产物主要集中于中部。对侧皮质少见c-fos阳性反应,视前区有轻度表达,纹状体呈中等强度的反应。缺血12h,c-fos阳性产物在上述区域开始减弱,24h强度明显减弱!高压氧治疗组皮质和视前区较单纯缺血组c-fos阳性产物强度明显减弱!缺血再灌注12h,高压氧治疗组视前区、纹状体中性白细胞数明显低于模型组(P<0.05);缺血再灌注24h,BACKGROUND：Hyperbaric oxygen （HBO） can increase oxygen diffusing capacity, thereby, improve hypoxic state of brain edema and brain tissue and promote the recovery of physiological function of brain cells in focal zone, the establishment of bypass circuit, and regeneration and repair of brain cells. OBJECTIVE： To observe the effect Jf hyperbaric oxygen on c-fos oncogene, expression of rats at different time points following acute focal cerebral ischemia/reperfusion（l/R） injury. DESIGN ： Randomized grouping animal experiment SETTING： Department of Emergency, Tangdu Hospital, Fourth Military Medical University of Chinese PLA; Department of Laboratory Medicine, Xi＇an Gaoxin Hospital;The General Hospital of the Air Force of Chinese PLA; Hyperbaric Oxygen Treatment Center, Department of Aerospace Medicine, Fourth Military Medical University of Chinese PLA. MATERIALS： This experiment was carried out in the Hyperbaric Oxygen Treatment Center, Department of Aerospace Medicine, Fourth Military Medical University of Chinese PLA in April 2002. Sixty-five 2-month-old healthy male SD rats. METHODS： The involved rats were randomized into： model group （n =20）, normal control group （n =5）, pure oxygen treatment group （n =20） and HBO treatment group （n =20）. In the model group, following the method of Koizumi et al, rat models of middle cerebral artery （MCA） ischemia were developed. In the normal control group, only occlusion of arterial blood flow was omitted; In the pure oxygen treatment group, the operation procedure was the same as that of model group, and embolus being drawn out at ischemia for 1 hour, rats were placed in the hyperbaric cabin at 2,9,21, 45 and 69 hours after embolus being inserted, and they inhaled pure oxygen under the normal pressure; In the HBO treatment group, the operation procedure was the same as that of model group, and rats inhaled pure oxygen for 1 hour under 025 MPa pressure. MAIN OUTCOME MEASURES： By means of immunohistochemical and pathohi

关 键 词：高压氧 局灶性脑缺血 FOS癌蛋白 血管通透性 脑 

分 类 号：R742[医药卫生—神经病学与精神病学]