基于多种敏感膜的多参数细胞微生理计研究  

Research on Multi-Parametric Micro-Biophysiometer Based on Multi-Sensitive Membranes

作　　者：余辉[1] 蔡华[1] 徐莹[1] 刘清君[1] 王平[1] 

机构地区：[1]浙江大学生物医学工程与仪器科学学院生物传感器国家专业实验室生物医学工程教育部重点实验室,杭州310027

出　　处：《微纳电子技术》2007年第7期367-369,共3页Micronanoelectronic Technology

基　　金：国家自然科学基金重点资助项目(30627002)

摘　　要：基于对多种离子敏感膜的研究,结合微透镜阵列,设计了一种基于光寻址电位传感器(LAPS)的新型多参数微生理计,实现同时并行检测H+,Na+,K+,Ca2+等多种不同离子。已经测得H+,K+,Ca2+-敏LAPS的灵敏度分别达到50mV/pH,43mV/pK以及25mV/pCa,线性度良好。A novel multi-parametric micro-biophysiometer based on light addressable potentiomet- ric sensor （LAPS） was designed. By depositing different ion-sensitive membranes on the surface of LAPS, combining with the micro-lens array system, the micro-biophysiometer can synchro- nously detect several different ions such as H^＋ , Na^＋ , K^＋ and Ca^2＋ quantitatively. The sensitivities of the H^＋ , K^＋ , and Ca^2＋-sensitive LAPS were measured to be 50 mV/pH, 43 mV/pK and 25 mV/pCa respectively with good linearity.

关 键 词：细胞传感器 光寻址电位传感器 微生理计 微透镜阵列 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]