基于电聚合蛋白A固定抗体的免疫微传感芯片  

Micro Immunosensor Based on Immobilizing Antibody with Electropolymerized Protein A

作　　者：边超[1] 孙辑舟[1] 夏善红[1] 

机构地区：[1]中国科学院电子学研究所传感技术联合国家重点实验室北方基地,北京100080

出　　处：《微纳电子技术》2007年第7期370-372,386,共4页Micronanoelectronic Technology

基　　金：国家自然科学基金资助项目(90307014)

摘　　要：采用MEMS工艺在硅衬底上制备了电极型免疫微传感芯片，重点探索了微传感器敏感表面抗体固定化的方法。尝试采用蛋白A与吡咯共同电化学聚合，进而由蛋白A结合抗体的方法，实现了抗体的固定。将该免疫传感器对人免疫球蛋白G（HIgG）进行检测，在5-160ng/mL以及160-640ng/mL呈分段线性的特性，实现了微剂量、低浓度的免疫检测，验证了该固定化方法的可行性。此法具有省时、简便、易控、适合微传感器固定的优点。An immunosensor of microelectrodes was fabricated on silicon wafer using MEMS technology. An antibody immobilization method suitable for micro scale was developed. Protein A and polypyrrole were electropolymerized, and antibody was immobilized by the oriented bond with protein A. Human immunoglobulin G （HIgG） was detected by this micro immunosensor with low concentration and small reagent usage. Two linear responses to HIgG concentrations from 5 ng/mL to 160 ng/mL, and from 160 ng/mL to 640 ng/mL, respectively, were displayed by this micro immunosensor. The feasibility of the antibody immobilization method was proved. This immobilization method shows many advantages, such as fast, simple, controllable and suitable for microsensor.

关 键 词：免疫微传感器 微机电系统 电化学聚合 聚吡咯 蛋白A 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]