南极生境真菌Gliocladium catenulatum T31菌株杀虫活性的研究  

A STUDY ON THE INSECTICIDAL ACTIVITY OF ANTARCTIC EPIPHYTE GLIOCLADIUM CATENULATUM T31

作　　者：牛德庆[1] 田黎[1] 周俊英 郑立[1] 陈靠山[3] 

机构地区：[1]国家海洋局第一海洋研究所现代化学分析室,青岛266061 [2]青岛城阳区农业局,青岛266109 [3]山东大学生命学院,济南250100

出　　处：《极地研究》2007年第2期131-138,共8页Chinese Journal of Polar Research

基　　金：国家"863计划"(2007AA09Z435);国家自然科学基金(20602009)资助

摘　　要：本文对影响南极生境真菌Gliocladium catenulatum T31生长和杀虫活性的初始pH值、发酵时间、培养温度等影响因子进行了研究。并以小菜蛾为试虫,以校正死亡率为评价指标,利用微型平板法对真菌T31的杀虫活性进行测试,发酵液杀虫活性最高为60.42%。发酵物乙酸乙酯提取后,丙酮溶解0.33mg/mL浓度下,发酵液提取物杀虫活性最高为82.05%,菌体提取物杀虫活性为76.92%。盆栽试验证明该菌株发酵液对小菜蛾第7d的虫口减退率为54.2%。In this study, the effects of major environmental factors on the growth and insecticidal activity of antarctic fungi Gliocladium catenulatum T31 were investigated. The method of microplate was employed. The bioactivity of the strain T31 to Diamondback Moth （Plutella xylostella） was studied with insect mortality as evaluated index. The most insecticidal activity of T31 fermentation broth was 60.42%. After acetic ether extracted T31 fermentation broth repeatedly, dissolved by acetone at the concentration of 0.33mg/mL, the best insecticidal activity of T31 fermentation broth extracts was 82.05% and insecticidal activity of T31 mycelium extracts was 76.92%. The potted experimentation showed that the controlling effect of T31 fermentation broth to Diamondback Moth was 54.2% on the seventh day.

关 键 词：南极真菌 杀虫活性 小菜 蛾盆栽试验 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]