脑梗死家兔尿激酶溶栓后滴注巴曲酶的作用及安全性研究  

Study of effects and safety of transfused Batroxobin after using Urokinase in rabbit with acute cerebral infarction

作　　者：季建中[1] 李新军[1] 曹保森[1] 杨志峰[1] 李冬梅[1] 侯惠敏[1] 李银[1] 翟江[1] 高风英 唐景旺 

机构地区：[1]河北北方学院附属第二医院,河北宣化075100 [2]河北省三河市妇科医院,河北三河102800 [3]河北省三河市医院,河北三河102800

出　　处：《现代中西医结合杂志》2007年第27期3949-3950,共2页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：河北省科学技术研究与发展指导计划项目(编号072761440)

摘　　要：目的研究急性脑梗死家兔尿激酶溶栓后滴注巴曲酶的安全性及对脑组织超氧化物歧化酶、丙二醛的影响。方法雄性健康新西兰大白兔18只,随机分为对照组、治疗1组、治疗2组,每组6只,采用颈内动脉自血栓塞法建立脑梗死模型。造模后2 h静脉溶栓。造模后24 h处死动物观察脑组织病理变化,测定脑组织超氧化物歧化酶活性、丙二醛含量。结果治疗1组、治疗2组各有2例出血;治疗1组2例有梗死灶,治疗2组仅1例有梗死灶;治疗2组超氧化物化歧酶活性最高,对照组最低,3组之间均有显著性差异(P均<0.05);2个治疗组丙二醛含量下降,与对照组比较有显著性差异(P<0.05)。结论急性脑梗死家兔尿激酶溶栓后滴注巴曲酶可以增强自由基清除能力而不增加出血发生率。Objective It is to study the safety of transfused Batroxobin after using Urokinase in rabbit with acute cerebral infarction and the influence to SOD and MDA. Method 18 male healthy New Zealand rabbit were randomizedly divided into three groups： model control group, group 1 of therapy and group 2 of therapy. Each group contained 6 animals. The rabbit model was established by using self embolism to embolize the cerebral artery through intracervical artery, Thrombolysis treatment through vein began at 2 hours after model formation. The histopathological changes of the brain tissue and the changes of SOD and MDA in the brain tissue were observed through head cutting at 24 hours after model formation. Results There were 2 cases of brain hemorrhage both in therapy groups. 2 cases had focus in group 1 of therapy; 1 case had focus in group 2 of therapy. The activity of SOD was the highest in group 2 of therapy and the lowest in model control group, There were difference samong three groups （P 〈 0.05）. The contents of MDA of both therapy groups were lower than that of the model group （ P 〈 0.05 ）, Conclusion Transfusing with Batroxobin after using Urokinase in rabbit with acute cerebral infarction can improve clear ability of the free radical, but doesn＇t increase the incidence of haemorrhage.

关 键 词：脑梗死 尿激酶 巴曲酶 超氧化物化酶 丙二醛 

分 类 号：R743.3[医药卫生—神经病学与精神病学]