Glypican-3蛋白在肝细胞癌患者血清和组织中的表达及意义  

Glypican-3 protein expression in serum and tissues and its clinical significance in hepatocellular carcinoma

作　　者：缪辉来[1] 谢贵林 温继育[1] 陈念平[1] 陈明[1] 包仕廷[1] 

机构地区：[1]广东医学院附属医院肝胆外科,广东省湛江市524001 [2]宁波市第二人民医院,浙江省宁波市315000

出　　处：《世界华人消化杂志》2007年第21期2311-2315,共5页World Chinese Journal of Digestology

基　　金：广东省第二批科学事业费计划项目;No.2004B30301001;湛江市科技招标项目;No.20040025

摘　　要：目的:探讨肝细胞癌(HCC)患者血清和组织中Glypican-3(GPC3)蛋白的表达及临床意义.方法:收集HCC患者27例和肝良性病变患者28例的术前1d空腹血清及组织标本,采用Western blot检测血清中GPC3蛋白,同时采用免疫组织化学SP法检测组织标本中GPC3的表达情况.结果:27例HCC患者HCC组织、癌旁组织和远癌肝组织中GPC3蛋白阳性的表达率分别为81.5%,0%和0%(HC:23.4689,P<0.001),术前血清中GPC3蛋白阳性率分别为55.6%;28例肝良性病变患者组织和血清中阳性率均为0%;GPC3蛋白对HCC诊断的敏感性为55.6%,特异性为100%,误诊率为0%.HCC患者血清中GPC3蛋白表达与其瘤体大小和病理分级之间差异有显著性(P<0.05),而与患者年龄、性别、HBsAg及AFP值之间差异无显著性(P>0.05).结论:GPC3蛋白在HCC患者血清和组织中有较高的表达,对诊断HCC有较高的敏感性和特异性,可作为HCC早期诊断的标志物.AIM： To investigate glypican-3 （GPC3） protein expression and its clinical significance in serum and tissues in hepatocellular carcinoma （HCC）. METHODS： Serum and tissues from 27 patients with HCC and 28 with benign liver disease were collected the day before operation. Expression of GPC3 in serum was detected by Western blotting, and expression in tissues was detected by immunohistochemistry. RESULTS： In the 27 patients with HCC, GPC3 expression in the HCC, paracarcinomatous and non-tumor liver tissues was 81.5, 0 and 0%, respectively （HC： 23.4689, P 〈 0.001）, and that in the serum before operation was 55.6%. There was no expression of GPC3 in the tissue and serum of the 28 patients with benign liver disease. The sensitivity, specificity and false-positive rates were 55.6, 100 and 0%, respectively, with serum GPC3 protein standard alone in the diagnosis of HCC. GPC3 protein expression in serum had no correlation with patient age or sex, alfa feta protein and hepatitis B surface antigen of HCC （P 〉 0.05）, but was correlated with tumor size and pathological grade （P 〈 0.05）. CONCLUSION： GPC3 protein expression in serum and tissue in HCC is higher than in non-HCC tissue. There is also greater sensitivity and specificity, and over-expression of GPC3 in HCC serum, which may be of value for the early diagnosis of HCC.

关 键 词：肝细胞癌 GLYPICAN-3 基因表达 诊断 

分 类 号：R735.7[医药卫生—肿瘤]