胃癌组织RASSF1基因启动子区甲基化的意义  

Methylation of the RASSFIA gene promoter in gastric carcinoma

作　　者：李学彦[1] 傅宝玉[1] 

机构地区：[1]中国医科大学附属一院消化科,辽宁省沈阳市110003

出　　处：《世界华人消化杂志》2007年第21期2354-2357,共4页World Chinese Journal of Digestology

摘　　要：目的:探讨抑癌基因RASSF1A启动子在胃癌组织及胃良性病变中甲基化的发生情况及与临床特征的关系.方法:采用甲基化特异性聚合酶链反应(MS- PCR)方法检测32例胃癌组织及32例相应的癌旁组织,以及46例胃良性病变中RASSF1A基因甲基化发生情况.结果:在32例胃癌DNA标本中,RASSF1A基因甲基化发生率为62.5%(20/32).而在32例癌旁组织中,只有1例存在甲基化,占3.1%(1/32),二者之间比较差异有统计学意义(P<0.05).在胃良性病变,浅表性胃炎和萎缩性胃炎中甲基化发生频率分别为3.3%和37.5%.RASSF1A基因甲基化发生率与胃癌分化程度、肿瘤大小及淋巴结转移之间无显著相关性.结论:RASSF1A基因启动子区甲基化在胃癌的发生发展中起重要作用.AIM： To investigate the methylation status of the suppressor gene RASSF1A promoter in gastric carcinoma and benign gastric disease, and its clinicopathological significance. METHODS： Methylation status of the RASSF1A promoter was detected by methylation specific-polymerase chain reaction （MS-PCR） in 32 patients with gastric carcinoma, adjacent non-carcinomatous gastric tissues, and 46 patients with benign gastric disease. RESULTS： The frequency of RASSF1A promoter methylation in gastric carcinoma was 62.5% （20/32）, and in adjacent non-carcinomatous gastric tissues, it was 3.1% （1/32）, while in chronic shallow gastritis and chronic atrophic gastritis, it was 3.3% （1/30） and 37.5% （6/16）, respectively. The rates of RASSF1A promoter methylation in gastric carcinoma and adjacent non-carcinomatous gastric tissues were significantly correlated （P 〈 0.01）. Methylation of RASSF1A promoter in gastric carcinoma had no relationship with differentiation grade, lymph node metastasis and tumor size.CONCLUSION： Methylation of RASSF1A promoter in gastric carcinoma may be an important molecular change and contribute to the development of gastric carcinoma.

关 键 词：胃癌 RASSF1A 抑癌基因 甲基化特异性聚合酶链反应 

分 类 号：R735.2[医药卫生—肿瘤]