新基因HERV-H-X的env区分析及其在结肠癌组织中的表达  

Deletion of the env Region in HERV-H-X Gene and Its Expression in Colon Cancer

作　　者：梁巧仪[1] 许则丰[1] 徐荣臻[1] 郑树[1] 丁佳逸[1] 

机构地区：[1]浙江大学附属第二医院肿瘤研究所,浙江杭州310009

出　　处：《癌症》2007年第9期952-956,共5页Chinese Journal of Cancer

基　　金：国家自然科学基金资助项目(No.30471943)~~

摘　　要：背景与目的:我们在结肠癌组织中发现了一个新的基因HERV-H-X。本研究的目的是分析HERV-H-Xenv区的缺失情况,研究其与其他HERV-Henv开放阅读框(open reading frames,ORF)的表达关系以及其在结肠癌与正常组织中的表达差异情况。方法:对HERV-H-X与含完整的envORF的HERV-H/env62、HERV-H/env60以及HERV-H/env59进行多序列比对,分析HERV-H-Xenv区的缺失情况。对HERV-H-X设计特异的引物,对HERV-H/env62、env60以及env59的envORF设计共同的引物,分别在8对结肠癌与相应癌旁正常组织中进行RT-PCR,比较它们的表达情况。对HERV-H-X进行实时荧光定量PCR,研究其在17对结肠癌与相应正常组织中的表达水平。结果:HERV-H-Xenv区缺失的序列正好相应于HERV-H/env62、env60以及env59的envORF区。RT-PCR结果显示HERV-H-X特异地在结肠的癌组织中表达,而HERV-Henv的ORF在结肠的癌组织与正常组织中都有表达。定量PCR结果显示HERV-H-X在结肠癌组织的平均表达水平是相应正常组织的24.9倍,经F检验证实差异有统计学意义(P<0.01)。结论:HERV-H-X的表达与结肠癌特异相关,但由于缺失env的ORF,其在结肠癌的高表达与env基因无关。BACKGROUND ＆ OBJECTIVE： We have identified a novel gene HERV-H-X from colon cancer tissue recently. This research was to analyze the deletion of the env region of HERV-H-X, to compare its expression with that of the env open reading frames （ORFs） in other HERV-H proviruses, and to study its differential expression in colon cancer and normal colon tissues. METHODS： Multiple alignments were performed for HERV-H-X and 3 other HERV-H proviruses （HERV-H/env62, HERV-H/env60, and HERV-H/env59） containing intact env ORF. HERV-H-X-specific polymerase chain reaction （PCR） and env ORF-common PCR for HERV-H/env62, env60 and env59 were conducted to study their expression in 8 pairs of colon cancer and adjacent normal tissues. Real-time quantitative PCR was conducted to study the differential expression of HERV-H-X in 17 pairs of colon cancer and adjacent normal tissues. RESULTS： The missing part in the env region of HERV-H-X corresponded to env ORFs in HERV-H/env62, HERV-H/env60 and HERV-H/env59. HERV-H-X was only detected in colon cancer tissues, while env ORFs were detected both in cancer and normal tissues. The expression of HERV-H-X was up-regulated in colon cancer tissues by 24.9 folds of that in normal tissues （P〈0.01）. CONCLUSION： HERV-H-X is highly expressed in colon cancer, but its up-regulation is not related to env gene as it lacks an env ORF.

关 键 词：人内源性逆转录病毒 差异表达 基因 聚合酶 链反应 实时定量 

分 类 号：R735.35[医药卫生—肿瘤]