子宫颈上皮内瘤样病变和子宫颈癌组织中Smad2/3和HPV16 E7的表达及意义  

Expression and Significance of Smad2/3 and HPV16 E7 in Cervical Intraepithelial Neoplasia and Cervical Carcinoma

作　　者：田媛[1] 吴鹏[1] 罗爱月[1] 奚玲[1] 周剑锋[2] 马丁[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院妇产科,湖北武汉430030 [2]华中科技大学同济医学院附属同济医院血液科,湖北武汉430030

出　　处：《癌症》2007年第9期967-971,共5页Chinese Journal of Cancer

基　　金：国家自然科学基金(No.30500596);国家重大基础研究项目(No.2002CB513107)~~

摘　　要：背景与目的:Smads蛋白是转化生长因子-β(transforming growth factor-β,TGF-β)超家族信号转导的下游信号蛋白,与多种肿瘤的发生密切相关;人乳头状瘤病毒(human papilloma virus,HPV)感染是宫颈癌的重要致癌因素,但目前对两者在宫颈癌发病过程中相互关系的研究尚不充分。本研究拟检测不同宫颈病变组织中Smad2/3和HPV16E7蛋白的表达,探讨HPV感染和Smad2/3蛋白变化在宫颈癌形成和演进进程中的相互关系。方法:采用免疫组织化学SP法检测20例宫颈慢性炎症、30例宫颈上皮内瘤样病变(cervical intraepithelial neoplasia,CIN)和30例宫颈癌组织中Smad2/3和HPV16E7的表达情况,比较其在各种病变中表达水平的差异。结果:Smad2/3蛋白在慢性宫颈炎、CIN和宫颈癌组织中的阳性率分别为50.0%、73.3%和93.3%,宫颈癌组分别与慢性宫颈炎组和CIN组比较,差异有统计学意义(P<0.05),慢性宫颈炎组与CINⅢ级组相比,差异有统计学意义(P<0.05)。HPV16E7蛋白阳性率在慢性宫颈炎、CIN和宫颈癌组织中分别为60.0%、66.7%和83.3%,各组间两两比较差异无统计学意义(P>0.05)。Smad2/3阳性率与宫颈癌临床分期、病理类型、组织学分级和淋巴结转移无关(P>0.05)。Smad2/3与HPV16E7表达呈正相关(r=0.271,P=0.015)。结论:Smad2/3蛋白在宫颈癌组织中表达升高,可能在宫颈癌的发生过程中起重要作用;HPV感染与Smad2/3蛋白在宫颈癌组织中表达升高密切相关。BACKGROUND ＆ OBJECTIVE： Smad proteins are downstream signal proteins of the transforming growth factor-β （TGF-β） superfamily, which are intimately related to the genesis of many human carcinomas. Human papillomavirus （HPV） is an important carcinogenic agent of cervical cancer. However, their relationship in the genesis of cervical cancer is unclear. This study was to detect the expression of Smad2/3 and HPV16 E7 protein in different cervical lesions, and to explore their possible roles in tumor genesis and progression. METHODS： The expression of Smad2/3 and HPV16 E7 in 20 specimens of chronic cervicitis, 30 specimens of cervical intraepithelial neoplasia （CIN）, and 30 specimens of cervical cancer was detected by SP immunohistochemistry. RESULTS： The positive rates of Smad2/3 were significantly lower in cervicitis and CIN than in cervical cancer （50.0% and 73.3% vs. 93.3%, P〈0.05）, and significantly lower in cervicitis than in CINⅢ（P〈0.05）. The positive rates of HPV16 E7 were 60.0% in cervicitis, 66.7% in CIN, and 83.3% in cervical cancer （P〉0.05）. The expression of Smad2/3 in cervical cancer had no correlation to clinical stage, pathologic classification, histological grade, and lymph node involvement（P〉0.05）. Smad2/3 expression was positively correlated to HPV16 E7 infection （r=0.271, P=0.015, R-Spearman）. CONCLUSIONS： Overexpression of Smad2/3 may be involved in the genesis of cervical cancer. It is closely correlated to HPV infection in cervical carcinoma.

关 键 词：宫颈肿瘤 宫颈上皮内瘤样病变 SMAD2 SMAD3 HPV16 E7 

分 类 号：R737.33[医药卫生—肿瘤]