HPLC双波长梯度洗脱同时测定通塞脉微丸中绿原酸、阿魏酸、甘草苷的含量  

Determination of Chlorogenic Acid and Ferulic Acid and Liquiriitin in Tong Sai Mai Tinypil by Dual Wavelength High-Performance Liquid Chromatography with Gradient Elution

作　　者：池玉梅[1] 崔小兵[1] 陈维[1] 李伟[1] 狄留庆[1] 

机构地区：[1]南京中医药大学,南京210029

出　　处：《中国药学杂志》2007年第17期1348-1350,共3页Chinese Pharmaceutical Journal

基　　金：国家863计划资助项目(2003AA2Z3247);江苏省自然基金资助项目(Bk2005150)

摘　　要：目的研究同时测定通塞脉微丸中绿原酸、阿魏酸及甘草苷含量的方法。方法KromasilC18色谱柱,乙腈-2%冰醋酸为流动相,梯度洗脱,检测波长λ1=324nm,λ2=276nm。结果绿原酸、阿魏酸、甘草苷分别在0.2084～3.334、0.05035～0.8056,0.2315～3.704μg内有良好的线性关系,r>0.9999;平均加样回收率分别为99.07%,100.40%,98.13%,相应的RSD分别为0.73%,1.32%,0.97%(n=6)。结论本法操作简便,结果可靠,重现性好,可作为产品质量控制的方法。OBJECTIVE To establish a method tor the determination of chlorogenic acid and ferulic acid and liquiriitin in Tong Sai Mai tinypil. METHODS The separation was performed on a Kromasil C18 column with the mobile phase consisting of acetonitrile-2% acetic acid for gradient elution. Chlorogenic acid, ferulic acid and liquiriitin were determined by dual wavelength of 324 nm（ λ1 ） and 276 nm（ λ2 ）. RESULTS The linearities were in the ranges of 0. 208 4-3. 334 μg for chlorogenic acid, 0. 050 35 - 0. 805 6μg for ferulic acid, and 0.231 5-3. 704 μg for liquiriitin, respeetively. The average recoveries was 99.07% （ HSD =0. 73% n =6）, 100.40% （RSD=l.32% n=6）, 98.13% （RSD=0.97% n=6）, respectively. CONCLUSION This method is convenient, rapid and accurate. It ca, be used for quality control in production of Tong Sai Mai tinypill.

关 键 词：通塞脉微丸 绿原酸 阿魏酸 甘草苷 高效液相色谱法 

分 类 号：R969.1[医药卫生—药理学]