蛇毒神经生长因子对体外培养鼠视网膜神经节细胞的保护作用  

Protective effects of venom nerve growth factor on cultured rat retinal ganglion cells in vitro

作　　者：杜秀娟[1] 刘金华[1] 钱道卫[1] 邓秋琼[1] 雷丹青[2] 

机构地区：[1]广西医科大学第一附属医院眼科,广西自治区南宁市530021 [2]广西医科大学药学院蛇毒研究所

出　　处：《眼科新进展》2007年第9期644-648,共5页Recent Advances in Ophthalmology

基　　金：国家自然科学基金资助(编号:30060081;30460138);教育部春晖计划基金资助~~

摘　　要：目的研究蛇毒神经生长因子（venom nerve growth factor，vNGF）对离体培养的视网膜神经节细胞（retinal ganglion cells，RGCs）的存活和轴突再生的影响。方法采用新生大鼠视网膜神经细胞体外原代培养技术，加入不同浓度的vNGF，通过检测细胞的活力选择一个最佳浓度进行培养，行抗Thy-1．1免疫细胞化学染色，观察RGCs在体外的存活时间,比较实验组和对照组的RGCs个数、胞体直径和轴突长度。结果vNGF的最佳浓度是80μg.L，用该浓度作用于细胞，实验组与对照组细胞存活时间分别为13～14d和7～8d，轴突长度分别为（111.78±10.65）μm和（78.73±8.23）μm。阳性细胞个数分别为（224.23±3.83）个和（182.47±2.79）个。结论vNGF能促进体外视网膜神经细胞和RGCs的存活以及轴突的伸长，有视神经保护作用。Objective To study the neuroprotective effects of venom nerve growth factor（vNGF） on survival and regeneration of cultured rat retinal gangli on cells （RGCs） in vitro. Methods Neonatal rat retinal neurons were cocultured with various concentration of vNGF to gain the most efficient concentration. MTT chromatometry was used to assess the viability of retinal neurons. RGCs were first identified immunocytochemically with antibody against Thy 1.1.Then the survival time of RGCs,the number of RGCs,axonal length and so ma diameters of RGCs were observed under microscope. Results The most efficient concentration of vNGF is 80 μg . L. RGCs could survival for 13 ~ 14 days in DMEM containing vNGF, the RGCs could survival for 7 ~ 8 days in DMEM without vNGF. The axonal length of RGCs in experimental group and in the control were （111.78±10.65 ）μm and （78.73 ± 8.23 ）μm respectively. The number of RGCs in experimental group and in the control were 224.23 ± 3.83 and 182.47±2.79 respectively. Conclusion vNGF can enhance the viability of retinal neurons and ganglion cells and promote the survival and regeneration of RGCs in vitro.

关 键 词：蛇毒神经生长因子 视网膜神经节细胞 视神经保护 细胞培养 

分 类 号：R774.6[医药卫生—眼科]