产类细菌素乳酸乳球菌V528的紫外线诱变育种研究  

Studies on UV mutational breeding of Lactococcus lactis V528 for producing bacteriocin-like substance

作　　者：陈丽园[1] 姜洁[1] 陈晓琳[1] 张明[1] 

机构地区：[1]安徽农业大学生命科学学院,合肥230036

出　　处：《中国微生态学杂志》2007年第5期411-412,416,共3页Chinese Journal of Microecology

基　　金：安徽省自然科学基金(070413136);安徽省高校"十五"优秀人才计划(【教秘人】2004-100)

摘　　要：目的获取高产抑制鸡白痢沙门菌生长的类细菌素乳酸乳球菌的突变菌株。方法在紫外线照射致死率为82.7%的剂量下,对处于对数生长后期的乳酸乳球菌V528进行紫外线二次复合诱变处理。分别随机对第一次和第二次复合处理后生长的117株和310株菌进行效价测定分析。结果在实验所用的照射剂量下,抑菌效价提高的正突变菌株占有一定优势,负突变菌株较少,其中突变菌株Lact.174的抑菌效价较V528的效价提高了8.48倍,并具有一定的遗传稳定性。结论运用紫外诱变育种技术可有效地获得高产类细菌素的突变菌株。Objective To select mutants with high productivity of anti-Salmonella pullorum bacterioein-like substance. Methods The cells of Lactococcus lactis V528, in the late exponential phase, were mutated by ultraviolet radiation with 82.7% death rate. The colonies of 117 and 310 were seleted for testing the antibacterial titer of the culture supernarant, after radiated by the first time and sencond time respectively. Results Under this condition, the numbers of positive mutants had some content predominance over negative ones. And among them, the bacterioein-like substance yield of strain Lact. 174 was 8.48 times more than the original strain with stable inheritance. Conclusion Mutants with high productivity of bacterioein-like substance were got efficiently by UV mutational breeding.

关 键 词：乳酸乳球菌 类细菌素 觜外线诱变 

分 类 号：Q935[生物学—微生物学]