木霉菌T05-1对几种作物病原菌的抑制作用及发酵条件研究  

Inhibition Function to Pathogen of Serval Crops and Fermentation Condition

作　　者：肖淑芹[1] 薛春生[1] 姜晓颖[1] 庄敬华[1] 曹远银[1] 

机构地区：[1]沈阳农业大学植物保护学院农业部北方农作物病害免疫重点开放实验室,沈阳110161

出　　处：《沈阳农业大学学报》2007年第4期518-521,共4页Journal of Shenyang Agricultural University

基　　金：农业部948项目(201056);"十五"辽宁省科技攻关项目(2001208001)

摘　　要：为鉴定木霉菌T05-1种类,明确其生防机制和发酵条件,采用形态学鉴定、抑菌试验和液体发酵的方法,对分离自辽宁省锦州市蔬菜根际土壤的的木霉菌T05-1进行了鉴定、抑菌作用及发酵条件的研究。结果表明:木霉菌T05-1经形态学鉴定为绿色木霉(Trichoderma viride Pers.ex Fr.),对病原菌的生防机制分别属于重寄生、抗生等作用,碳源为葡萄糖时T05-1菌丝生长最快,碳源为蔗糖时产孢量最大,氮源为蛋白胨和硫酸铵时产孢量最大,氮源为硝酸铵菌丝生长最快,温度为25℃,pH值为6～7有利于木霉菌T05-1的生长及产孢。因此绿色木霉T05-1是一株生防作用较好的菌株。The study was to identify Trichoderma 05-1, determine biocontrol mechanism and fermentation condition. Identification, inhibition function and fermentation condition of T05-1 collected from Jinzhou, Liaoning Province were studied with morphology identification and test of inhibition pathogens and fermentation. T05-1 was identified as morphotoglcally Trichoderma viride. Bi- parasitic and antibiotic mechanism were the main biocontrol mechanisms to different pathogen. Glucose of carbon source was the optimum mycelium growth of T05-1, the number of conidium was high on sucrose of carbon culture. Ammonium nitrate of nitrogen source was optimum mycelium for the growth of T05-1, the number of conidium was high on peptone and ammonium sulfate of nitrogen culture. 25℃ and pH=6-7 were the optimum conditions for myeelium growth and eonidium production of T05-1. TO5-1 has a good effect of biocontrol function.

关 键 词：木霉菌T05-1 生防机制 发酵条件 

分 类 号：S436.461[农业科学—农业昆虫与害虫防治]