乳腺癌组织中多药耐药相关基因产物的表达及其相互关系  

Studies on the expression and correlation of drug-resistance-associated genes in breast cancer tissues

作　　者：刘新兰[1] 王程[1] 王宁菊[1] 韩恩善[1] 秦璟[1] 

机构地区：[1]宁夏医学院附属医院,宁夏银川750004

出　　处：《宁夏医学杂志》2007年第9期779-781,共3页Ningxia Medical Journal

摘　　要：目的探讨多药耐药(MDR)基因产物P-糖蛋白(P-gp)、谷胱甘肽-S-转移酶-π(GST-π)及DNA拓扑异构酶Ⅱ(TopoⅡ)在乳腺癌组织中的表达及其相互关系。方法采用免疫组织化学方法,检测184例未经抗肿瘤治疗的原发性乳腺癌组织中P-gp、GST-π及TopoⅡ表达水平。结果乳腺癌组织中P-gp、GST-π和TopoⅡ阳性表达率分别为49.51%、45.65%和73.91%,TopoⅡ阳性表达率明显高于P-gp及GST-π阳性表达率(P<0.05);2种多药耐药基因产物共表达率为30.98%,2种和3种多药耐药基因产物共表达率为67.39%,明显高于单独表达率12.41%(P<0.01);P-gp与GST-π的表达呈显著正相关(P<0.05),P-gp和TopoⅡ的表达呈明显负相关(P<0.05),GST-π和TopoⅡ的表达呈明显负相关(P<0.05)。结论P-gp、GST-π及TopoⅡ在乳腺癌组织中表达对肿瘤耐药起重要作用,单基因和多基因协同作用,以多基因共表达为主。Objective To explore expression of muhidrug resistance （MDR） gene production of P - glycoprotein （ P - gp）, Glntathione - s - transferase - π （ GST - π） and Topo II in breast cancer tissue. Methods A total of 184 case with untreated primary breast carcinoma were detected immunohistochemically for P - gp, GST - π and Topo II. Results The positive expression rate of P - gp, GST - π and Topo II separately were 49.51% ,45.65% and 73.91%. The positive expression rate of Topo II was much higher than that of P- gp,GST-π （ P 〈 0.05）. The coexpression rate of only 2 MDR gene production and 3 MDR gene production was 30.98% and 67.7% , respectively. Those coexpression rates were higher than individual expression rate （ 12.41% ）. There was significantly pos- itive correlation beteen P - gp and GST - π （ P 〈 0.05） and there was significantly negative correlation beteen P - gp related and Topo II （P 〈 0.05 ）. GST - π had obviously negative correlation with Topo II （ P 〈 0.05 ）, Conclusion The expression of P - gp, GST -π and Topo 11 in breast cancer tissue play an important role in tumor drug - resistance. There is synergistic action between single gene and multiple genes.

关 键 词：乳腺肿瘤 多药耐药 P-糖蛋白 谷胱甘肽-S-转移酶-Π 拓扑异构酶Ⅱ 

分 类 号：R737.9[医药卫生—肿瘤]