神经生长因子对局灶性脑缺血再灌注大鼠顶叶皮质tau蛋白磷酸化的影响  

Effect of NGF on tau hyperphosphorylation in rat parietal cortex after focal cerebral ischemia-reperfusion

作　　者：张正洪[1,2] 席刚明[3] 李文春[1] 黄铁柱[1] 张一飞[1] 曲鹏[4] 方秀斌[4] 

机构地区：[1]郧阳医学院解剖学教研室 [2]郧阳医学院附属人民医院神经内科,湖北十堰442000 [3]郧阳医学院附属人民医院神经内科 [4]中国医科大学基础医学院神经生物学教研室

出　　处：《中国现代医学杂志》2007年第17期2069-2072,2077,共5页China Journal of Modern Medicine

基　　金：辽宁省自然科学基金资助项目(No:619019)

摘　　要：目的探讨外源性神经生长因子(NGF)对局灶性脑缺血再灌注大鼠顶叶皮质神经元tau蛋白过度磷酸化的影响。方法用线栓法制作局灶性脑缺血再灌注模型,应用免疫组织化学SABC法、Western Blotting和图像分析方法检测大鼠缺血侧顶叶皮质tau蛋白在Ser199/202位点磷酸化程度和总tau蛋白表达。结果缺血再灌注组顶叶皮质tau蛋白在Ser199/202位点磷酸化水平和总tau蛋白表达比假手术组显著升高(P<0.05);NGF组大鼠顶叶皮质tau蛋白在Ser199/202位点磷酸化水平显著低于缺血再灌注组和高于假手术组,NGF组总tau表达也下降但仍高于假手术组(P<0.05)。结论NGF明显减轻局灶性脑缺血再灌注大鼠顶叶皮质tau蛋白磷酸化程度,NGF可能通过降低tau蛋白磷酸化水平对缺血神经元起保护作用。[Objective] To explore the effect of exogenous nerve growth factor （NGF） on tau hyperphosphorylation in rat parietal cortex during focal cerebral ischemia/reperfusion （I/R）. [Methods] Focal cerebral ischemia/reperfusion model was induced by occlusion of the fight middle cerebral artery using the intraluminal suture method. The level of tau hyperphosphorylation at Ser199/202 site and total tau protein in rat fight parietal cortex during focal cerebral ischemia/reperfusion were detected by SABC immunohistochemical method and Western Blotting, and the positive products were analyzed by image analysis system. [Results] The level of tau hyperphosphorylation at Ser199/202 site and total tau in rat ipsolateral parietal cortex were significantly higher in I/R group than sham group （P 〈0.05）. NGF significantly inhibited tau hyperphosphorylation at Ser199/202 site and the level of total tau was also signiticanfly lower in NGF group than that in I/R group, but they were still higher that in sham group（P 〈0.05）. [Conclusions] NGF can reduce the level of tau hyperphosphorylation in rat parietal cortex during focal cerebral ischemia/reperfusion and inhibiting tau hyperphosphorylation may be involved in the protective mechanism of NGF to ischemic neuron.

关 键 词：脑缺血 神经生长因子 TAU蛋白 磷酸化 大鼠 

分 类 号：R743[医药卫生—神经病学与精神病学] R-332[医药卫生—临床医学]