卵巢癌患者瘤组织相关基因编码蛋白表达和DNA倍性与肿瘤转移的关系  

The relationship between oncogene coded protein expression and DNA ploidy measurement in tumor tissue and tumor metastasis from patients with ovarian cancer

作　　者：秦宇 周振英[2] 

机构地区：[1]广东省深圳市盐田区妇幼保健院,广东深圳518081 [2]江苏省肿瘤防治研究所

出　　处：《中国医师杂志》2007年第8期1050-1052,共3页Journal of Chinese Physician

摘　　要：目的探讨卵巢癌患者肿瘤组织相关基因编码蛋白表达与肿瘤转移的关系。方法采用流式细胞术对32例卵巢癌患者肿瘤组织的p53V＋、CD44v5＋、CD44v6＋、cerbB-2＋、nm23＋细胞百分率和DNA含量进行了检测和分析。结果卵巢癌患者p53V＋、CD44v5＋、CD44v6＋、cerbB-2＋的百分率显著升高（P〈0.01或P〈0.05），肿瘤转移者进一步升高；nm23＋细胞表达率却显著降低（P〈0.05），肿瘤转移时进一步降低（P〈0.05）。肿瘤组织DNA倍体类型不同，p53V＋、CD44v5＋、CD44v6＋、cerbB-2＋、nm23＋细胞表达率明显不同。结论卵巢癌患者肿瘤组织肿瘤相关基因编码蛋白表达率、DNA倍性和肿瘤转移之间的关系十分密切。它们可以作为卵巢癌患者病情发展、转移、肿瘤恶性度和患者预后判断的重要指标。Objective To approach the relationship between oncogene coded protein expression in tumor tissues and tumor metastasis tissues from patients with ovarian cancer. Methods The percentage of p53V ＋ , CD44v5 ＋, CD44v6 ＋, cerbB-2 ＋, nm23 ＋ cells and DNA content in the tumor tissues from 32 patients with ovarian cancer were measured and analyzed by flow cytometry. Results The percentage of p53V ＋ ,CD44v5 ＋ ,CD44v6 ＋ and cerbB-2 ＋ cells in tumor tissues were significantly higher than those in normal control （ P 〈0. 01 or P 〈 0. 05 ）. In tumor metastasis tissues, these parameters were gradually increased. The percentage of nm23 ＋ cells in tumor tissues was significantly lower than that in normal control （ P 〈0. 05 ）. In tumor metastasis tissues, this parameter was gradually decreased. These parameters and DNA ploidy types in tumor tissue were significant different. Conclusions There is a marked relationship among the expression rate of oncogene coded protein, DNA ploidy in tumor tissues and tumor metastasis tissues in patients with ovarian cancer. Therefore, these parameters may be regarded markers of development, metastasis, malignant degree and prognosis of ovarian cancer.

关 键 词：卵巢肿瘤 肿瘤转移 DNA 肿瘤 倍性 蛋白质P53 抗原 CD44 基因 ERBB-2 

分 类 号：R737[医药卫生—肿瘤]