HPLC法测定复方α酮酸片中五种氨基酸的含量  

Determination of amino acids of compound α-keto acid tablets by HPLC with 2,4-dinitrofluorobezene derivation

作　　者：胡世林[1] 丁文忠[2] 

机构地区：[1]安徽省立医院,安徽合肥230001 [2]安徽中医学院,安徽合肥230032

出　　处：《安徽医药》2007年第9期797-798,共2页Anhui Medical and Pharmaceutical Journal

摘　　要：目的建立复方α酮酸片中5种氨基酸的含量测定方法。方法以2,4-二硝基氟苯（FDNB）为衍生化试剂,将片剂中的氨基酸定量衍生化,衍生化条件为在碱性溶液中60℃暗处反应1 h,制得5种DNP-氨基酸。色谱柱为Luna C18（250 mm×4.6mm,5μm）;以甲醇-醋酸盐缓冲液系统梯度洗脱;检测波长为360 nm。结果衍生化产物性质稳定;5种氨基酸在各自的浓度范围内线性关系良好（r〉0.999 0）;加样回收率为97.2%-102.8%,相对标准偏差均小于2.0%。结论该方法准确,简便,重现性好,专属性强,可用于该产品的质量控制。Aim To develop a method for determination of amino acids of compound α-keto acid tablets. Methods Derivated by 2 ,4- dinitrofluorobenzene,5 kinds of DNP-amino acids in the tablets were analyzed by RP-HPLC with an Luna C18 column（4.6 mm ×250 mm,5 μm） as stationaryphase,methanol/acetate buffer as mobile phase,The detection wavelength was at 360 nm and the flow rate was 1.0 ml ·min^-1. Results The average recovery of all amino acids were 97.2% - 102.8% and RSD of all amino acids were 2.0 %. Conclusion The method is simple, accurate, reliable and can be used for determination of amino acids in compound α-keto acid tablets.

关 键 词：柱前衍生化 2 4-二硝基氟苯 氨基酸 高效液相色谱法 

分 类 号：R927[医药卫生—药学]