腰带长体茧蜂Macrocentrus cingulum毒液和卵巢蛋白的电泳分析  

The SDS-PAGE analysis of venom and ovarian proteins from Macrocentrus cingulum

作　　者：李永[1] 陆剑锋[2] 柯欣[1] 符文俊[1] 

机构地区：[1]中国科学院上海生命科学研究院植物生理生态研究所,上海200032 [2]合肥工业大学生物与食品工程学院,合肥230009

出　　处：《昆虫天敌》2007年第3期103-107,144,共6页Natural Enemies of Insects

基　　金：国家自然科学基金面上项目(NSF30370195)

摘　　要：寄生蜂毒液和卵巢蛋白在寄生过程中起着重要作用,蛋白成分和性质的研究日益深入。本文主要通过SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)试验,分析了腰带长体茧蜂毒液和卵巢蛋白的分子量组成。结果表明,腰带长体茧蜂毒液蛋白图谱显示约有7条蛋白带介于43～100kDa之间,含量较高的三条带为97kDa、64kDa、45kDa;卵巢蛋白图谱显示约有12条蛋白带,位于30～200kDa之间,含量较高的两条带为39kDa、43kDa。并对毒液和卵巢的生物学功能进行了探讨。The venom and ovarian proteins from parasitoid wasp play an important role in parasitization. The protein components and characteristics from these two apparatus have been increasingly deeply researched. By the method of the SDS-PAGE we analyzed the components of venom and ovarian proteins from Microcentrus cingulum. The results showed that there were seven bands among 43 - 100kDa in venom and the abundant proteins were 97kDa, 64kDa and 45kDa. Twelve bands among 30 -200kDa existed in the ovarian proteins and the abundant proteins were 39kDa and 43kDa. The biological functions of venom and ovarian proteins were also discussed.

关 键 词：腰带长体茧蜂 毒液 卵巢蛋白 SDS-PAGE 

分 类 号：S476.3[农业科学—农业昆虫与害虫防治]