诱导型一氧化氮合酶和血管内皮生长因子在舌癌细胞株中的表达  

Expression of Inducible Nitric Oxide Synthase and Vascular Endothelial Growth Factor in Tca8113 Cells

作　　者：曾曙光[1] 陈伟良[2] 杨岚[2] 邱钧琦[1] 张积仁[3] 章锦才[1] 艾伟健[1] 郑俊发[1] 

机构地区：[1]广东省口腔医院.南方医科大学附属口腔医院,广东广州510280 [2]中山大学附属第二医院,广东广州510120 [3]南方医科大学珠江医院肿瘤中心,广东广州510282

出　　处：《中山大学学报（医学科学版）》2007年第5期520-523,共4页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：广东省自然科学基金(032573);广东省科技计划项目(2005B30901014);广东省卫生厅科研基金(A2005113)

摘　　要：【目的】探讨诱导型一氧化氮合酶(iNOS)和血管内皮生长因子(VEGF)在舌鳞癌细胞株Tca8113中的表达,为进一步研究iNOS和VEGF在舌鳞癌肿瘤血管生成中的作用提供实验基础。【方法】采用逆转录-聚合酶链反应(RT-PCR)法和SP免疫组化方法,对舌鳞癌细胞株Tca8113中iNOS和VEGF mRNA和蛋白表达情况进行检测。【结果】RT-PCR检测到舌鳞癌Tca8113细胞株中iNOS\VEGF mRNA条带明显,SP免疫组化检测到iNOS\VEGF蛋白在舌癌细胞胞浆中呈强阳性表达。【结论】iNOS\VEGF mRNA和蛋白在舌鳞癌Tca8113细胞株中呈高水平表达。[Objective] To investigate the expression of inducible nitric oxide synthase （iNOS） and vascular endothelial growth factor （VEGF） in Tca8113 and provide basement of experiment for further study on the role of iNOS and VEGF in tongue carcinoma angiogenesis. [Methods] iNOS and VEGF mRNA and protein were detected by RT-PCR and SP immunohistochemistry. [Results] The band of iNOS and VEGF mRNA were obvious and the expression of iNOS and VEGF protein were strongly positive by immunohistochemistry in Tca8113 cells. [ Conclusion ] There were overexpression of iNOS and VEGF mRNA and protein in Tca8113cells.

关 键 词：诱生型一氧化氮合酶 血管内皮生长因子 舌 肿瘤 

分 类 号：R739.2[医药卫生—肿瘤]