内向整流性钾通道4.1在人星形细胞瘤中的表达变化研究  

Alterations of the Expression of Inwardly-rectifying Potassium Channel 4.1 Protein in Human Astrocytic Tumors

作　　者：谭戈[1] 孙善全[1] 冉建华[1] 孙晓川[2] 肖虹[3] 宋彧[4] 刘科 

机构地区：[1]重庆医科大学基础医学院神经生物学实验室,重庆市400016 [2]重庆医科大学附属第一医院神经外科 [3]第三军医大学西南医院神经外科 [4]重庆医科大学附属第二医院神经外科 [5]重庆市急救中心神经外科

出　　处：《中国肿瘤临床》2007年第17期978-981,共4页Chinese Journal of Clinical Oncology

基　　金：国家自然科学基金资助(编号:30500171)

摘　　要：目的:研究内向整流性钾通道4.1(kir4.1)在人星形细胞瘤和正常脑组织的表达差异,探讨星形细胞瘤增殖、生长的分子机制。方法:选取50例星形细胞瘤患者的肿瘤组织,其中胶质母细胞瘤21例,间变性星形细胞瘤15例,星形胶质细胞瘤14例。以肿瘤周围相对正常脑组织作为对照。利用免疫组化与蛋白印迹(Western-blot)方法检测肿瘤组织与对照组织的kir4.1表达。结果:不同星形细胞瘤组织与对照组相比较,kir4.1表达增强(P<0.05);其中,恶性程度较高的胶质母细胞瘤相对于恶性程度较低的星形胶质细胞瘤、间变性星形细胞瘤,kir4.1表达更为强烈(P<0.05)。结论:kir4.1表达的变化与肿瘤的恶性程度有关。kir4.1在人星形细胞瘤组织表达增强。Objective：To study the alterations in the expression of inwardly-rectifying potassium channel 4.1（kir4.1）in human astrocytic tumors and to explore the molecular mechanism of astrocytic tumor proliferation and growth.Methods：The tumor tissues of 50 patients with astrocytic tumors were selected,including 21 cases of glioblastoma,15 cases of anaplastic astrocytoma and 14 cases of astrocytoma.The normal brain tissue around the tumor served as a control.Immunohistochemical staining and Western blot analysis were used to measure the expression of kir4.1 protein.Results：The expression of kir4.1 was significantly increased in tumor tissues compared with the control samples（P〈0.05）,and the expression of kir4.1 in glioblastoma was much higher than that in astrocytoma and anaplastic astrocytoma.Conclusion：Protein expression of kir4.1 is related to the malignancy of astrocytic tumors and it is increased in human astrocytic tumors compared to normal controls.

关 键 词：星形细胞瘤 钾通道 内向整流 

分 类 号：R739.4[医药卫生—肿瘤]