缓激肽对C6胶质瘤细胞IL-1β表达的影响  

Effects of bradykinin on the expression of interleukin-1β in C6 glioma cells

作　　者：王振华[1] 薛一雪[2] 

机构地区：[1]中国医科大学基础医学院生理学教研室,辽宁沈阳110001 [2]中国医科大学基础医学院神经生物教研室,辽宁沈阳110001

出　　处：《中国药理学通报》2007年第9期1174-1176,共3页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30670723;30600060;30400145;30570650);辽宁省自然科学基金资助项目(No20052102);高等学校博士学科点专项科研基金资助项目(No20050159005)

摘　　要：目的探讨缓激肽对大鼠C6胶质瘤细胞内白细胞介素-1β(IL-1β)表达水平的影响,为进一步研究缓激肽开放血肿瘤屏障的机制提供实验依据。方法采用逆转录-聚合酶链反应(RT-PCR)半定量技术和放射免疫法分别检测缓激肽作用C6胶质瘤细胞后,胶质瘤细胞IL-1β mRNA及细胞培养液中IL-1β的含量。结果RT-PCR结果显示缓激肽作用15min后,IL-1β mRNA的表达水平较对照组升高(P<0.05),然后逐渐下降;放射免疫法结果亦证明了C6胶质瘤细胞在缓激肽作用15min后,其细胞培养液中IL-1β的含量最高。结论缓激肽可刺激大鼠C6胶质瘤细胞中IL-1β的分泌增强,IL-1β的表达上调可能在缓激肽增加血肿瘤屏障通透性的过程中发挥一定作用。Aim To investigate the effects of bradykinin on the expression of IL-1β in C6 glioma cells and provide an experimental basis for the mechanism of bradykinin opening the blood tumor barrier.Methods Semi-quantitive RT-PCR and radioimmunoassay were used to detect the expression of IL-1βmRNA in C6 glioma cells and the content of IL-1β in the culture media treated with bradykinin respectively.Results RT-PCR revealed that the expression of IL-1βmRNA was significantly elevated with bradykinin treated for 15min（P〈0.05）.Subsequently,it was attenuated gradually.Radioimmunoassay also revealed that the content of IL-1β in the cell culture media was highest with bradykinin treated for 15 min.Conclusion The secretion of IL-6 can be increased in C6 glioma cells induced by bradykinin,which may play an important role in the process of bradykinin augmenting the permeability of the blood tumor barrier.

关 键 词：神经胶质瘤 缓激肽 白细胞介素-1Β 血肿瘤屏障 

分 类 号：R-332[医药卫生] R329.24