GP与抗癌药物合用对肿瘤多药耐药的逆转作用  

The reversal of P-glycoprotein-mediated MDR by GP associated with chemotherapeutic drugs

作　　者：徐宏彬[1] 何玲[1] 刘国卿[1] 李海涛[2] 王成章[3] 

机构地区：[1]中国药科大学药理学教研室,江苏南京210009 [2]南京中医药大学药理学教研室,江苏南京210009 [3]中国林科院林产化学工业研究院,江苏南京210004

出　　处：《中国药理学通报》2007年第9期1207-1212,共6页Chinese Pharmacological Bulletin

基　　金：江苏省自然科学基金资助项目(NoBK2004110)

摘　　要：目的研究GP与抗癌药物合用对肿瘤多药耐药的逆转。方法MTT法测定GP与抗癌药物合用对K562/DOX和A549/DOX细胞的细胞毒作用;流式细胞术测定GP对K562/DOX细胞内阿霉素累积的影响。结果GP与阿霉素合用,能够明显逆转K562/DOX细胞对阿霉素的耐药性,并且能够浓度依赖性增加K562/DOX细胞内阿霉素的剂量;GP与阿霉素、顺铂、长春新碱合用时,对A549/DOX耐药性逆转作用相对较弱。结论GP能促进抗癌药物在MDR细胞内的累积,增强抗癌药物对MDR细胞的细胞毒作用,逆转肿瘤多药耐药。Aim To investigate the reversal effect of GP on the activity of P-gp in MDR-associated tumor cells and to develop a useful approach to inhibit the drug efflux in MDR-associated tumor cells.Methods MTT assay was used to detect the effect of GP on the cytotoxicity of cisplatin（DDP）,vincristine（VCR） and doxorubicin（DOX）.Flow cytometry was used to determine the influence of GP on the intracellular accumulation of DOX.Results GP synergistically increased the cytotoxity and the intracellular accumulation of DOX in K562/DOX cells;GP also increased the cytotoxity of DDP,DOX,and VCR in A549/DOX cells.However,the effect was relatively weak.Conclusion GP could increased the cytotoxity and the intracellular accumulation of chemotherapeutic drugs in MDR-associated tumor cells.GP may be a promising MDR modulator.

关 键 词：银杏聚戊烯醇 P-糖蛋白 肿瘤多药耐药 K562/DOX A549/DOX 

分 类 号：R-329.24[医药卫生] R73-351