谷氨酸和γ-氨基丁酸对原代培养星形胶质细胞神经甾体合成释放的影响  

Effects of GLU and GABA on the genesis of neurosteroids in primary cultured astrocytes

作　　者：侯艳宁[1] 江平[1] 吴红海[1] 张昊[1] 

机构地区：[1]中国人民解放军白求恩国际和平医院药学部,河北石家庄050082

出　　处：《中国药理学通报》2007年第9期1227-1230,共4页Chinese Pharmacological Bulletin

摘　　要：目的研究氨基酸类神经递质对原代培养大鼠大脑皮质星形胶质细胞神经甾体合成释放的影响。方法采用原代培养的大鼠大脑皮质星形胶质细胞,分别加入不同浓度的谷氨酸和γ-氨基丁酸处理48h;采用固相萃取结合高效液相色谱质-谱联用分析方法提取分离和测定细胞培养液中游离型(脱氢表雄酮,DHEA;孕烯醇酮,PREG;别孕烯醇酮,AP)及结合型神经甾体(脱氢表雄酮硫酸酯,DHEAS;孕烯醇酮硫酸酯,PREGS)。结果与生理盐水对照组比较,谷氨酸处理使PREG和PREGS水平明显下降,DHEAS水平明显升高;γ-氨基丁酸处理使PREG水平明显降低,AP水平增加。结论谷氨酸和γ-氨基丁酸两种神经递质对原代培养的星形胶质细胞PREG合成释放均呈抑制作用;谷氨酸对DHE-AS、γ-氨基丁酸对AP的合成释放分别呈现明显促进作用;高剂量的谷氨酸还可以抑制PRGES的合成和释放。Aim To investigate effects of glutamate（GLU） and γ-aminobutyric acid（GABA） on levels of neurosteroids synthesized and released by primary cultured rat brain cortical astrocytes.Methods Primary cultured rat brain cortical astrocytes were treated with GLU or GABA for 48 h respectively.Unconjugated（dehydroepiandrosterone,DHEA;pregnenolone,PREG;allopregnanolone,AP） and conjugated neurosteroids（dehydroepiandrosterone sulfate,DHEAS;pregnenolone sulfate,PREGS） in culture media were extracted by solid phase extraction（SPE） and analyzed by HPLC-MS.Results Compared with saline control group,GLU treatment decreased PREG and PREGS levels and increased DHEAS level significantly;GABA treatment decreased PREG level and increased AP level significantly.Conclusion Both GLU and GABA were shown to inhibit the genesis of PREG.While GLU significantly promoted the genesis of DHEAS and GABA promoted the genesis of AP.The genesis of PREGS was also inhibited by GLU treatment in primary cultured cortical astrocytes.

关 键 词：谷氨酸 Γ-氨基丁酸 星形胶质细胞 神经甾体 液相色谱-质谱 

分 类 号：R-332[医药卫生] R322.81