变链菌葡糖基转移酶遗传多态性与乳牙高龋的关系  

A primary study on the relationship between genetic diversity within glucosyltransferases of streptococcus mutans and caries in deciduous teeth

作　　者：林焕彩[1] 支清蕙[1] 杨军英[2] 廖义东[1] 张蓉[1] 

机构地区：[1]中山大学光华口腔医学院口腔预防医学教研组,广州510055 [2]中山大学附属第一医院口腔科

出　　处：《现代口腔医学杂志》2007年第5期455-458,共4页Journal of Modern Stomatology

基　　金：广东省自然科学基因资助项目(04009344)

摘　　要：目的对乳牙高龋与变链菌葡糖基转移酶基因遗传多态性的关系进行初步研究。方法以20名高乳牙龋幼儿合成水不溶性葡聚糖量最高的15个菌株及20名无龋幼儿合成水不溶性葡聚糖量最低的15个菌株作为实验菌株,UA159为参考菌株,应用三种限制性内切酶(HinfⅠ、TaqⅠ、MboⅠ)以PCR-RFLP法分析高龋无龋幼儿变链菌临床分离株葡糖基转移酶gtfBC基因的遗传多态性。结果HinfⅠ的酶切图谱在两组幼儿间存在差别,高龋组有5株与UA159一致,而无龋组3株与UA159一致,但差别没有统计学意义;TaqⅠ酶切图谱除无龋组有一株与UA159不同外,其余均相同;所有MboⅠ的酶切图谱与UA159相同。结论高龋和无龋幼儿gtfBC基因限制性酶切图谱未发现有统计学意义的差异。Objective To make a primary research on the connection between genetic diversity within glucesyltransferases of Streptococcus mutans （ S. mutans ） and high dmft of children. Methods Fifteen S. mutans strains which synthesized the highest amount of water-insoluble glucan from 20 children with high dmft were chosen as case group and 15：3. rnutans strains which synthesized the lowest amount of water-insoluble glucan from 20 cariesfree children were chosen as the control. S. rnutans UA159 was used as referenced strain. The genetic diversity of gtfBC from the DNA of isolates was assessed by PCR - RFLP method with restriction endonucleases： Hinf I , Taq I and Mbo I . Results There were 5 isolates in caries-active group and 3 isolates in caries-free group had the same Hinf I digestion files with UA159, but no significant differences were found between the two groups. Taq I digestion files in the gtfBC of clinical isolates were all the same except for one isolates from caries-free group. No difference was observed in the Mbo I digestion files. Conclusion No significant difference has been found for the charts of gtfBC of S. mutans between children with high dmft and no caries.

关 键 词：乳牙 龋病 变形链球茵 gtfBC PCR-RFLP 

分 类 号：R788.1[医药卫生—口腔医学]