端粒酶反转录酶活化与p53金属蛋白酶-9表达对非小细胞肺癌发生的影响  

Influence of the activation of human telomerase reverse transcriptase and the expression of p53 and matrix metalloproteinase-9 on non-small cell lung cancer

作　　者：杨廷桐[1] 李秀杰[1] 武俊芳[1] 张艳芳[1] 李磊[1] 刘欣欣[1] 

机构地区：[1]新乡医学院病理学教研室,河南新乡453000

出　　处：《新乡医学院学报》2007年第5期443-447,共5页Journal of Xinxiang Medical University

基　　金：河南省科委攻关科研基金资助项目(编号:001170632)

摘　　要：目的探讨人端粒酶反转录酶(hTERT)、p53、金属蛋白酶-9(MMP-9)与非小细胞肺癌(NSCLC)发生的内在联系及其作用。方法应用原位杂交、HE及免疫组织化学技术对104例NSCLC组织进行检测,探讨NSCLC发生的分子机制及诊断的可能性。结果(1)hTERT mRNA、p53、MMP-9在NSCLC中均有较高的表达,阳性率分别为73.08%,69.23%,84.62%。(2)hTERT mRNA在鳞癌中表达为64.71%,腺癌中为88.89%(2χ=6.997,P<0.01)。(3)p53与hTERT mRNA阳性表达呈正相关性(r=0.651,P<0.05),二者的阳性共表达为53.85%,一致性表达为65.40%。p53与MMP阳性表达呈正相关性(r=0.477,P<0.05),二者的阳性共表达为53.85%,一致性表达为53.85%。hTERT mRNA与MMP-9阳性表达呈正相关性(r=0.671,P<0.01),二者阳性共表达率为71.15%,一致性表达率为75.00%。结论p53、hTERT mRNA、MMP-9具有协同促进NSCLC发生的作用;hTERT mRNA的表达与NSCLC的类型有关。Objective To investigate the relationship and effect of human telomerase reverse transeriptase（hTERT）,p53 and matrix metalloproteinase-9（MMP-9） to human non-small cell lung cancer（NSCLC）.Methods The expression of hTERT mRNA、 p53 and MMP-9 were detected by in situ hybridization,HE and SP immunohistochemistry in 104 cases of NSCLC.The mechanism of occurrence of NSCLC and the possibility of diagnosis were study.Results（1） The expression of hTERTmRNA,p53 and MMP-9 in NSCLC was high,and the positive rates were 73.08%,69.23% and 84.62% respectively.（2）The positive rates of hTERT mRNA in squamous cell carcinoma and adenocarcinoma were 64.71% and 77.78% respectively（χ^2 =6.097,P〈0.01）.（3）A positive correlation was present between p53 and hTERTmRNA protein expression（r=0.651,P〈0.05）,both positive coexpression was 53.85%,expressd regularity was 65.40%.A positive correlation was present between p53 and MMP-9 protein expression（r=0.447,P〈0.05）,both positive coexpression was 53.85%,expressd regulation was 53.85%.A pos itive correlation was present between hTERTmRNA and MMP-9 protein expression（r=0.671,P〈0.01）.Both positive expression was 71.15%,expressed regularity was 75.00%.Conclusion hTERT mRNA,p53 and MMP-9 have synergy of promoting the lung cancer carcinogenesis,the expression of hTERT mRNA was related with the type of NSCLC.

关 键 词：非小细胞肺癌 人端粒酶反转录酶 P53 金属蛋白酶-9 

分 类 号：R363.1[医药卫生—病理学]