ODC反义RNA对人肺鳞癌细胞c-myc及c-Ha-ras基因表达的影响  

EFFECT OF ODC ANTISENSE RNA ON EXPRESSION OF C MYC AND C Ha RAS ONCOGENES IN HUMAN LUNG SQUAMOUS CARCINOMA CELLS

作　　者：关钧[1] 范慕贞 

机构地区：[1]临床医学研究所生化及分子生物学室

出　　处：《中日友好医院学报》1997年第1期3-5,共3页Journal of China-Japan Friendship Hospital

基　　金：国家自然科学基金

摘　　要：目的：探讨鸟氨酸脱羧酶（ＯＤＣ）反义ＲＮＡ逆转人肺鳞癌细胞ＬＴＥＰ７８（Ｌ７８）恶性表型的分子机理。方法：核酸杂交，免疫细胞化学染色等。结果：在ＯＤＣ反义基因稳定整合、ＯＤＣ反义ＲＮＡ高效表达的转染细胞株中，ｃｍｙｃ及ｃＨａｒａｓｍＲＮＡ的表达比Ｌ７８亲本细胞显著下降；ｒａｓＰ２１蛋白的表达也受到明显抑制。结论：ＯＤＣ反义ＲＮＡ引起细胞内多胺耗竭，对ｃｍｙｃ及ｃＨａｒａｓ基因表达有明显的下调作用，这可能是其逆转人肺鳞癌细胞恶性表型的重要机制之一。Objective:The molecular mechanisms of ornithine decarboxylase(ODC) antisense RNA reversing malignant phenotypes of human lung squamous cell carcinoma LTEP 78(L 78 )were further studied.Methods:Nucleic acid hybridization and immunocytochemical staining.Results:The content of c myc and c Ha ras mRNA in cells transfected by ODC antisense RNA was much lower than that in L 78 parental cells,and that the expression of ras P 21 protein was also markedly inhibited.Conclusion:Polyamine deprivation caused by ODC antisense RNA downregulated the expression of c myc and c Ha ras oncogenes,which might be one of the molecular mechanisms of malignant phenotype reversion of the antisense transfectants.

关 键 词：鸟氨酸脱羧酶 反义核糖核酸 肺鳞癌细胞 

分 类 号：R734.202[医药卫生—肿瘤]