Dynamic Proteome Changes of Shigella flexneri 2a During Transition from Exponential Growth to Stationary Phase  

作　　者：Li Zhu Xian-Kai Liu Ge Zhao Yi-Dan Zhi Xin Bu Tian-Yi Ying Er-Ling Feng Jie Wang Xue-Min Zhang Pei-Tang Huang Heng-Liang Wang 

机构地区：[1]State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology, Beijing 100071, China [2]National Center of Biomedical Analysis, Beijing 100850, China

出　　处：《Genomics, Proteomics & Bioinformatics》2007年第2期111-120,共10页基因组蛋白质组与生物信息学报（英文版）

基　　金：This work was supported by the National Key Basic Research Program of China （973 Program, No. 2005CB522904） ;the National Natural Science Foundation of China （No. 30470101）.

摘　　要：Shigella flexneri is an infectious pathogen that causes dysentery to human, which remains a serious threat to public health, particularly in developing countries. In this study, the global protein expression patterns of S. flexneri during transition from exponential growth to stationary phase in vitro were analyzed by using 2-D PAGE combined with MALDI-TOF MS. In a time-course experiment with five time points, the relative abundance of 49 protein spots varied significantly. Interestingly, a putative outer membrane protein YciD （OmpW） was almost not detected in the exponential growth phase but became one of the most abundant proteins in the whole stationary-phase proteome. Some proteins regulated by the global regulator FNR were also significantly induced （such as AnsB, AspA, FrdAB, and KatG） or repressed （such as AceEF, OmpX, SodA, and SucAB） during the growth phase transition. These proteins may be the key effectors of the bacterial cell cycle or play important roles in the cellular maintenance and stress responses. Our expression profile data provide valuable information for the study of bacterial physiology and form the basis for future proteomic analyses of this pathogen.Shigella flexneri is an infectious pathogen that causes dysentery to human, which remains a serious threat to public health, particularly in developing countries. In this study, the global protein expression patterns of S. flexneri during transition from exponential growth to stationary phase in vitro were analyzed by using 2-D PAGE combined with MALDI-TOF MS. In a time-course experiment with five time points, the relative abundance of 49 protein spots varied significantly. Interestingly, a putative outer membrane protein YciD （OmpW） was almost not detected in the exponential growth phase but became one of the most abundant proteins in the whole stationary-phase proteome. Some proteins regulated by the global regulator FNR were also significantly induced （such as AnsB, AspA, FrdAB, and KatG） or repressed （such as AceEF, OmpX, SodA, and SucAB） during the growth phase transition. These proteins may be the key effectors of the bacterial cell cycle or play important roles in the cellular maintenance and stress responses. Our expression profile data provide valuable information for the study of bacterial physiology and form the basis for future proteomic analyses of this pathogen.

关 键 词：Shigella flexneri PROTEOMICS growth phase 

分 类 号：Q949.3[生物学—植物学]