离体培养的小麦花药对镰刀菌毒素反应之研究  

Response of in Vitro Wheat Anther to Fusarium graminearum Toxin

作　　者：陈耀锋[1] 任惠莉[1] 韩德俊[1] 李春莲[1] 崔传斌 

机构地区：[1]西北农业大学农学系

出　　处：《西北农业学报》1997年第2期23-26,共4页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家九五攻关项目和杨陵科学基金

摘　　要：通过不同浓度的镰刀菌毒素对离体培养的小麦花药进行胁迫。结果表明，随着胁迫浓度的增加，小麦花药培养效率迅速下降，具体表现在降低培养花药的反应率、愈伤组织诱导率、绿苗分化率及相对增加白苗率四方面。毒素的这种效应，因供试材料基因背景的不同而有一定差异，相对而言。Response of in vitro wheat anther to Fusarium graminearum toxin was studied using induction medium containing Fusarium graminearum crude toxin. The results showed that wheat anther culture efficiency fell down quickly with the concentration in creasingof the crude toxin in induction media. The effects of the toxin on wheat anther culture existed in such following aspects as reducing the response frequency of wheat anther, induction frequency anther callus and the number of green plantlets, and relatively adding the number of albino plantlets compared to the crude toxin free controls. The effect of crude toxin was different among variant genotype. Anthers from susceptible cultivars/hybrids were easier to be killed by lower toxin concentrations than those from cultivars/hybrids of less susceptible parents.

关 键 词：小麦 花药培养 镰刀菌毒素 培养效率 

分 类 号：S512.103.5[农业科学—作物学]