HPLC-MS^n法研究次乌头碱在水中的化学反应  

Chemical reaction of hypaconitine in water studied by HPLC-MS^n

作　　者：郗瑞云[1] 刘永刚[1] 张宏桂[1] 乔延江[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《北京中医药大学学报》2007年第8期539-542,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家重点基础研究发展规划项目(十五攻关)课题资助项目(No.2004BA721A11);国家自然科学基金资助项目(No.39870874)

摘　　要：目的研究次乌头碱在水中发生的化学反应。方法采用高效液相色谱-电喷雾-质谱/质谱(HPLC-ESI-MSn)方法,液相用梯度洗脱,质谱用正离子模式。结果在水解液中发现了4种水解产物,其准分子离子[M+H]+分别为574、470、498、556。结论通过对水解产物的准分子离子和碎片离子的分析,鉴定其水解产物为次乌头次碱、次乌头原碱、N-去甲基乙酰基次乌头原碱和焦次乌头次碱,并推测在水中发生脱乙酰基反应、脱乙酰基同时脱苯甲酰基反应、脱苯甲酰基同时脱N上的甲基反应、脱乙酰基同时脱水的反应。Objective To study the chemical reaction of hypaconitine in water. Method The method of HPLC-ESI-MSn was applied in gradient elution program and the mass spectrometry was in positive ion mode. Result There were 4 hydrolysates found in the hydrolytic solution and their quasi-molecular ion [M ＋ H ] ＋ were respectively at m/z 574, 470, 498 and 556. Conclusion Through the analysis on quasi-molecular ion and fragment ion, the hydrolysates of hypaconitine are identified as benzoylhypaconine, hypaconine, N-demethylacetylhypaconine and pyrhypaconitine. There are 4 reactions in water deduced in the procedure.

关 键 词：水解产物 次乌头碱 高效液相色谱-电喷雾-质谱/质谱法 

分 类 号：R284.2[医药卫生—中药学]