Anti-Tumor Effect of Heat Shock Protein 70-Peptide Complexes on A-549 Cells  

作　　者：王洪琦 胡晨霞 胡玲 申维玺 赵健 祁岩超 韩霞 李建国 张正 

机构地区：[1]Guangzhou University of Chinese Traditional Medicine [2]Guangzhou Tumor Hospital

出　　处：《Chinese Journal of Cancer Research》2007年第3期210-215,共6页中国癌症研究（英文版）

基　　金：a grant from the Natural Science Foundation of Guangdong Province, China (No. 04010022).

摘　　要：Objective： To investigate the anti-tumor immunity （HSP70-PC） from human lung cancer tissue. Methods in vitro of heat shock protein 70-peptide complexes HSP70-PC was purified from lung tumor tissues and corresponding non-tumor lung samples with the methods of ADP-affinity chromatography, DEAE ion-exchange chromatography and Western-blot. The activation and proliferation of PBMC induced by different HSP70-PC and tumor cytotoxic reactivity to A549 cells in vitro were measured by the MTT cell proliferation assay. Results： The purified HSP70-PC had a very high purity found by SDS-PAGE and Western-blot. Human lymphocytes were sensitized efficiently by HSP70 preparation purified from lung cancer tissues and a definite cytotoxicity to A-549 cells was observed. There was significant difference with HSP70-PC purified from lung cancer, compared with the control group （P〈0.001）. Conclusion： High purity of HSP70-PC could be achieved from tumor tissues in this study. HSP70-PC purified from human tumor tissues can induce anti-tumor immunity in vitro mainly implemented by eliciting CTL immunity.Objective： To investigate the anti-tumor immunity （HSP70-PC） from human lung cancer tissue. Methods in vitro of heat shock protein 70-peptide complexes HSP70-PC was purified from lung tumor tissues and corresponding non-tumor lung samples with the methods of ADP-affinity chromatography, DEAE ion-exchange chromatography and Western-blot. The activation and proliferation of PBMC induced by different HSP70-PC and tumor cytotoxic reactivity to A549 cells in vitro were measured by the MTT cell proliferation assay. Results： The purified HSP70-PC had a very high purity found by SDS-PAGE and Western-blot. Human lymphocytes were sensitized efficiently by HSP70 preparation purified from lung cancer tissues and a definite cytotoxicity to A-549 cells was observed. There was significant difference with HSP70-PC purified from lung cancer, compared with the control group （P〈0.001）. Conclusion： High purity of HSP70-PC could be achieved from tumor tissues in this study. HSP70-PC purified from human tumor tissues can induce anti-tumor immunity in vitro mainly implemented by eliciting CTL immunity.

关 键 词：HSP70-PC Tumor vaccine Lung carcinoma 

分 类 号：R734.2[医药卫生—肿瘤]