激动M_3受体对间隙连接蛋白43表达的影响  

Activation of cardiac M_3 receptor influences connexin 43 expression

作　　者：于海雁[1] 岳朋[1] 张勇[1] 赵文晓[1] 邢妍[1] 霍荣[1] 吕延杰[1] 杨宝峰[1] 

机构地区：[1]哈尔滨医科大学药学院药理学教研室

出　　处：《中国药理学通报》2007年第6期711-715,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金重点资助项目(No30430780);重大基础研究前期专项(No2004CCA06700)

摘　　要：目的探索激动心肌M3受体后间隙连接蛋白43的变化情况,进一步发现M3受体作为抗心律失常药物作用新靶点的作用机制。方法通过免疫组化、划痕标记荧光传输技术结合激光共聚焦显微镜和RT-PCR的方法,研究激动M3受体对间隙连接蛋白43表达的影响。结果证实激动M3受体可以改善细胞和细胞之间的通讯,改善病理条件下间隙连接蛋白43的重构,且其磷酸化水平在间隙连接蛋白的重构过程中发挥了重要作用,并且还发现激动M3受体可以恢复病理条件下下降的间隙连接蛋白43的mRNA水平。结论激动M3受体可以改善病理条件下间隙连接蛋白43的重构,并且其磷酸化形式在闰盘重构中发挥重要的作用。Aim We would find changes of the connexin 43＇s expression by the activation of M3 receptor in order to investigate the mechanism of M3 receptor as the target of antiarrhythmic agents in rat ventricular myocardium. Methods Immunostaining, Lucifer Yellow scrape loading and dye transfer, confocal microscopy and RT-PCR were employed to detect the expression of connexin 43 protein pretreated by choline. Results Activation of M3 receptor could alter intracellular communication capacity between adjacent cells and the connexin 43＇s remodeling under pathological conditions. At the same time the phosphorylation of connexin 43 played an important role. Activation of M3 receptor could inhabit the descending of the connexin 43 mRNA level under pathological conditions. Conclusion Activation of M3 receptor can alter the remodeling of connexin 43 under pathological conditions, at the same time the phosphorylation of connexin 43 played an important role in the remodeling of intercalated disks.

关 键 词：胆碱 M3受体 间隙连接蛋白43 心律失常 药物靶点 

分 类 号：R-332[医药卫生] R322.11