非促分裂型haFGF对小鼠脑缺血/再灌注损伤的保护作用  

Protective effect of non-mitogenic haFGF on cerebral ischemia-reperfusion injury in mice

作　　者：谭毓治[1] 蔡飒[1] 李校堃[2] 刘峰[1] 胡华良[1] 

机构地区：[1]广东药学院药理学教研室,广东广州510224 [2]暨南大学医药生物技术开发中心,广东广州510632

出　　处：《中国药理学通报》2007年第6期738-741,共4页Chinese Pharmacological Bulletin

基　　金：国家高技术研究发展计划(863计划)重大专项资助项目(No2001AA215131)

摘　　要：目的研究非促分裂型人类aFGF(nm-haFGF)对脑缺血/再灌注损伤的保护作用及初步机制。方法采用跳台法和Y型电迷宫法,观察nm-haFGF对脑缺血/再灌注损伤小鼠学习记忆的影响;测定进入脑组织的依文思蓝的多少,观察nm-haFGF对脑缺血/再灌注损伤小鼠血脑屏障损伤修复程度的影响;测定实验小鼠脑组织中NO含量,观察nm-haF-GF对脑缺血/再灌注损伤小鼠脑组织生化指标的影响。结果nm-haFGF能提高脑缺血/再灌注损伤小鼠的学习记忆功能,降低其脑组织依文思蓝和NO的含量。结论nm-haFGF可改善脑缺血/再灌注损伤小鼠的学习记忆功能,改善和减轻血脑屏障损伤程度,降低脑缺血/再灌注损伤引起的NO增高,对脑血管、脑神经组织具有保护效应。Aim To investigate the protective effect of non-mitogenic human acidic fibroblast growth factor （nm-haFGF） on cerebral ischemia-reperfusion injury in mice. Methods Cerebral ischemia-reperfusion model was made by ligating bilateral carotid for 20 minutes in mice. These mice were randomly divided into model group（ iv NS）, two doses of nm-haFGF （iv 25, 50 μg·kg^-1） groups, rhaFGF group （iv 50 μg·kg^-1） and shamoperated group. Step down test and Y-type electric maze were used to examine the effect of nm-haFGF on learning and memory of mice, then Even＇s Blue（EB） level and NO level in brain of these mice were measured. Results The nm-haFGF significantly decreased numbers of errors of mice in 5 min in step down test and in Y-type electric maze test; EB and NO levels in brain of these mice were lower than those of model group respectively. Conclusion The nm-haFGF can protect cerebral ischemia-reperfusion injury in mice.

关 键 词：非促分裂型人类aFGF 脑缺血保护作用 学习记忆 NO 

分 类 号：R-332[医药卫生] R322.81