建立HPLC-纳米铜网版印刷电极-电化学检测器测定透析液中葡萄糖含量的方法  

Establishing the method of measurement of glucose concentration in brain microdialysate by HPLC-NCSE100-ED

作　　者：王巍[1] 王丹巧[1] 

机构地区：[1]中国中医科学院西苑医院老年医学研究所,北京100091

出　　处：《中国药理学通报》2007年第6期830-831,共2页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助课题(No30371824)

摘　　要：目的建立快速测定透析液中葡萄糖含量的方法。方法应用高效液相-电化学检测器和纳米铜网版印刷电极流动注射分析法测定微透析技术取样所得透析液中葡萄糖含量。结果该法测定的葡萄糖标准曲线的相关系数r=0.9995。葡萄糖日内测定的变异系数CV为4%～7%。测得大鼠纹状体透析液中葡萄糖的含量为(2.4±0.8)mmol·L-1。表明方法可靠。结论HPLC-纳米铜网版印刷电极-电化学检测器在测定透析液中葡萄糖有一定的应用价值。Aim To establish the rapidly detecting method of glucose concentration in brain microdialysate. Method Using high-performance liquid chromatography （HPLC） with electrochemical detector （ED） and Nano-Copper screen-printed electrode （NCSE100）-Flow injection analysis the glucose concentration in brain microdialysate were detected. Result The related coefficient （r） of glucose standard curve was 0. 9995. The variation coefficient （CV） within a day was 4% - 7%. The striatum extracellular glucose concentration in rats was（2. 4 ± 0. 8 ） mmol·L^-1. These results indicated that our method was reliable. Conclusion Using HPLC with ED, NCSE100, and flow injection analysis the glucose concentration in brain microdialysate were detected, which has certain applied worth.

关 键 词：葡萄糖 微透析 HPLC-纳米铜网版印刷电极-电化学检测器 

分 类 号：R-332[医药卫生] R322.81