全反式维甲酸对急性早幼粒细胞白血病TNF和IL-6活性的影响  

Effect of all trans retinoic acid on TNF and IL-6 activity of acute promyelocytic leukemia

作　　者：申红[1] 任海全[2] 唐天华[2] 王俊伟[2] 汪运山[1] 姜国胜[2] 

机构地区：[1]济南市中心医院中心实验室,250013 [2]山东省医学科学院基础医学研究所山东省肿瘤免疫与中药免疫重点实验室

出　　处：《国际肿瘤学杂志》2007年第9期714-717,共4页Journal of International Oncology

摘　　要：目的检测急性早幼粒细胞白血病（APL）患者经过全反式维甲酸治疗后血清和白细胞胞质内肿瘤坏死因子（TNF）和白细胞介素-6（IL-6）活性水平的变化与意义。方法TNF活性应用传统的生物学结晶紫法测定。采用IL-6依赖细胞株MH60-BSF2进行IL-6的生物学活性的测定。半固体琼脂法检测白血病集落（L-CFU）和粒一巨噬细胞系集落（GM-CFU）数量变化。结果治疗前的APL患者血清TNF和IL-6活性水平均高于对照组的相应水平（P〈0．01）。白细胞（WBC）升高期的血清TNF和IL-6活性出现降低趋势，但只有IL-6的变化具有统计学意义（P〈0．05）。完全缓解后的APL患者血清TNF和IL-6活性明显下降（P〈0．05），但仍然高于正常对照的相应水平（P〈0．05）。治疗前WBC胞质TNF和IL-6活性平均水平明显高于对照组，尤其是WBC高峰时胞质TNF活性升高更加明显（P〈0．05），缓解时胞质TNF活性明显降低。但在治疗过程中胞质IL-6活性轻度降低，没有明显的统计学意义（P〉0．05）。相关性分析结果表明，血清TNF和IL-6活性的变化与APL患者外周血WBC数量无明显相关性（P〉0．05），血清TNF变化与骨髓L-CFU数量明显相关（P〈0．05）。结论全反式维甲酸治疗虽然影响APL患者血清TNF和IL-6水平，但与该组患者的外周血WBC数量变化无线性关系。Objective To detect the changes and significance of serum and cell plasma TNF and IL-6 in patients with acute promyelocytic leukemia （APL） before and after treatment with all-trans retinoic acid （ATRA）. Methods TNF activity was detected by traditional crystaviolet method. The MH60-BSF2 IL-6 dependant clone was used to measure the IL-6 activity. L-CFU and GM-CFU were evaluated by semi-solid agar culture. Results The level of serum TNF and IL-6 activity in APL patients was higher than that of controls before treatment with ATRA （ P 〈 0. 01 ） . During the white blood cell （WBC） augmentation period,both serum TNF and IL-6 activity decreased, however,only the change of IL-6 had statistical singnificance （ P 〈 0.05）. On the other hand , serum TNF and IL-6 decreased obviously just after the complete remission （ P 〈0.05） but were still higher than those of the controls（ P 〈0.05）. The level of WBC cytoplasm TNF and IL-6, espercially TNF was higher than that of controls （ P 〈 0. 05 ） before treatment with ATRA. During the WBC augmentation period, WBC cytoplasm TNF activity increased obviously （ P 〈 0. 05）. But during the treatment, WBC cytoplasm IL-6 activity decreased slightly, and threr was no statistical significance（ P 〉0. 05）. It was also suggested that serum TNF and IL-6 activity was not related to the variation of WBC numbers （P 〉 0. 05 ）, but serum TNF was related to the variation of L-CFU significantly （ P 〈0. 05）. Conclusion ATRA treatment can affect serum level of TNF or IL-6 in patients with APL , but neither TNF nor IL-6 has linear correlation with the variation of WBC number.

关 键 词：白血病 早幼粒细胞 急性 维甲酸 肿瘤坏死因子 白细胞介素-6 

分 类 号：R733[医药卫生—肿瘤]