血卟啉衍生物光动力效应杀伤人鼻咽癌耐药与非耐药细胞株的实验研究  

Effect of hematoporphyrin derivative mediated photodynamic therapy in human nasopharyngeal carcinoma cell line CNE2 and CNE2/DDP in vitro

作　　者：杨小民[1] 罗荣城[1] 马红京[2] 李黎波[1] 丁雪梅[1] 严晓[1] 吕成伟[1] 

机构地区：[1]南方医科大学南方医院肿瘤治疗中心,广东广州510515 [2]解放军534医院肿瘤科,河南洛阳471003

出　　处：《中国耳鼻咽喉颅底外科杂志》2007年第4期241-244,共4页Chinese Journal of Otorhinolaryngology-skull Base Surgery

基　　金：中国临床肿瘤学科学基金资助(基金号:Y-2005-0013)

摘　　要：目的观察血卟啉衍生物(hematoporphyrin derivative,HpD)光动力学效应(photodynamic therapy,PDT)对体外培养人鼻咽癌细胞株CNE2及其顺铂耐药株CNE2/DDP的生物作用;探讨PDT治疗多药耐药(mul-tidrug resistance,MDR)鼻咽癌的可行性。方法对体外培养的CNE2与CNE2/DDP细胞分别加入0.5、1.0、1.5、2.0、2.5、3.0、3.5、4.0、5.0μg/ml不同浓度的HpD,孵育4h,给予波长为630nm的半导体激光进行照射,照射的功率密度为20mW/cm2,照射能量密度分别为2、5、10、20J/cm2,继续孵育24h,用四甲基偶氮唑盐(MTT)比色法测定细胞存活率。结果血卟啉衍生物介导的光动力(HpD-PDT)对体外培养的人鼻咽癌CNE2和CNE2/DDP细胞均有杀伤作用,表现为HpD浓度、激光剂量的量-效依赖关系。激光能量为20J/cm2时,HpD对CNE2和CNE2/DDP细胞半数杀伤浓度分别为0.7μg/ml与1.5μg/ml,两者比较有统计学意义(P<0.01)。相同HpD浓度、激光剂量条件下,CNE2/DDP细胞存活率显著高于CNE2细胞(P<0.05)。结论HpD-PDT对CNE2和CNE2/DDP细胞均有杀伤作用,但CNE2/DDP细胞对其敏感性低于CNE2细胞,表明CNE2/DDP对HpD-PDT存在一定程度的交叉抗性,增加HpD浓度和增大激光剂量可以在一定程度上克服这种抵抗。Objective To investigate the potential use of hematoporphyrin derivative （HpD） for photodynamic therapy （PDT） in the nasopharyngeal carcinoma （NPC） cell line CNE2 and CNE2/DDP cultured in vitro. Methods CNE2 and CNE2/DDP cells were incubated in media contained various concentrations of HpD （0.5, 1.0, 1.5, 2. 0, 2. 5, 3. 0, 3. 5, 4.0 and 5.0 μg/ml） for 4 hours and illuminated with different light doses using a semiconductor laser at 630 um with an energy density of 2, 5, 10, 20 J/cm^2. We analyzed the survival rates of CNE2 and CNE2/DDP cells with MTT assay. After 24 hours incubation with Hpd-PDT. Results HpD-PDT could effectively kill the CNE2 and CNE2/DDP cells cultured in vitro. The killing degree was positively correlated with the concentration of HpD and the dosage of radiation. The IC50 of CNE2 and CNE2/DDP was 0. 7, 1.5 μg/ml respectively with significant difference （P〈0. 01）, when they were exposed to 20 J/cm^2. The survival rate of CNE 2 / DDP cells was higher than that of CNE 2 under the same HpD concentration and radiation dosage （ P 〈0. 05）. Conclusion HpD-PDT may effectively kill CNE2/DDP cells as well as CNE2 cells cultured in vitro,though CNE2/DDP cells are less sensitive to HpD-PDT than CNE2 cells. The cross-resistance of CNE2/DDP to HpD-PDT may be overcome by increasing HpD concentration and radiation dosage.

关 键 词：鼻咽肿瘤 血卟啉衍生物 光动力学疗法 CNE2 CNE2/DDP 多药耐药 

分 类 号：R739.6[医药卫生—肿瘤]