9-顺式维甲酸诱导HL-60细胞凋亡  

9 cis retinoic acid induces apoptosis in HL 60 cells

作　　者：曾辉[1,2] 赵相印[1,2] 马雅銮[1,2] 赵如冰[1,2] 刘复强[1,2] 杨敏[1,2] 鞠淑英[1,2] 王瑾[1,2] 高天祥[1,2] 

机构地区：[1]首都医科大学附属北京同仁医院 [2]首都医科大学分子生物学实验中心

出　　处：《中华血液学杂志》1997年第7期358-360,共3页Chinese Journal of Hematology

基　　金：北京市自然科学基金;首都医科大学基础与临床合作基金

摘　　要：目的：检测９－顺式维甲酸（９－ｃｉｓＲＡ）诱导ＨＬ－６０细胞凋亡的能力，并探讨其分子机制。方法：应用形态学观察、ＤＮＡ电泳、流式细胞仪分析检测细胞凋亡，应用流式细胞仪检测ＨＬ－６０细胞ｂｃｌ－２含量。结果：９－ｃｉｓＲＡ可以诱导ＨＬ－６０细胞在分化后发生典型的凋亡。在ＨＬ－６０细胞分化和凋亡的过程中ｂｃｌ－２含量逐渐下降。９－ｃｉｓＲＡ诱导ＨＬ－６０细胞凋亡能力和降低ｂｃｌ－２表达的能力均显著强于全反式维甲酸（Ａ－ＴＲＡ）。结论：９－ｃｉｓＲＡ具有诱导ＨＬ－６０细胞凋亡的作用。ｂｃｌ－２表达水平的降低可能是经诱导分化成熟的白血病细胞走向凋亡的重要条件。Objective:To investigate the apoptosis of HL 60 cells induced by 9 cis retinoic acid (9 cis RA),and illustrate the possible molecular mechanism.Methods:Apoptosis was detected by morphological observation,DNA electrophoresis and flow cytometric cell cycle analysis.Bcl 2 expression was detected by flow cytometry.Results:9 cis RA initiated apoptosis of HL 60 cells after inducing them irreversibly commited to differentiation.Bcl 2 was downregulated during the differentiation and apoptosis process.9 cis RA was more potent than all trans retinoic acid (ATRA) did in inducing terminal differentiation associated apoptosis and in downregulation of Bcl 2 expression. Conclusion:9 cis RA can induce apoptosis in HL 60 cells.Downregulation of Bcl 2 expression appears to play an important role in the apoptosis of the differentiated leukemic cells.

关 键 词：细胞凋亡 9-顺式维甲酸 IL-60细胞 白血病 

分 类 号：R733.705[医药卫生—肿瘤] R977.21[医药卫生—临床医学]