脂质体介导的腹腔内IL-2和IL-6基因疗法对淋巴瘤小鼠巨噬细胞的激活作用  

Activation of macrophages from lymphoma bearing mice by liposome mediated intraperitoneal IL 2 and IL 6 gene therapy

作　　者：王全兴[1] 曹雪涛[1] 王建莉[1] 章卫平[1] 马施华[1] 叶天星[1] 

机构地区：[1]上海第二军医大学免疫学教研室

出　　处：《中华血液学杂志》1997年第7期364-367,共4页Chinese Journal of Hematology

基　　金：国家自然科学基金优秀中青年人才专项基金

摘　　要：目的：探讨白细胞介素２（ＩＬ－２）和白细胞介素６（ＩＬ－６）基因疗法的抗肿瘤作用机制。方法：将脂质体包裹的ＩＬ－２基因和ＩＬ－６基因直接注射至ＥＬ－４淋巴瘤小鼠腹腔后，研究淋巴瘤小鼠的腹腔巨噬细胞（Ｍφ）在基因治疗后的功能变化。结果：腹腔内单独注射脂质体包裹的ＩＬ－２基因后，可明显提高Ｍφ的ＭＨＣＩａ的表达，增强Ｍφ的杀伤活性，并促进Ｍφ诱导白细胞介素１（ＩＬ－１）及肿瘤坏死因子（ＴＮＦ）的产生；腹腔内单独注射脂质体包裹的ＩＬ－６基因后，对Ｍφ也具有激活作用，但效果不及前者。二者联合后能非常显著地提高荷瘤小鼠的Ｍφ的上述功能。结论：腹腔内脂质体介导的ＩＬ－２基因和ＩＬ－６基因治疗能有效地解除淋巴瘤小鼠体内Ｍφ功能的抑制，激活Ｍφ发挥抗肿瘤作用。Objective:To investigate the antitumor mechanism of interleukin 2 (IL 2) and interleukin 6(IL 6) gene therapy.Methods:IL 2 DNA liposome and IL 6 DNA liposome were i.p injected into EL 4 lymphoma bearing mice. The function of macrophages from the mice was assayed.Results:The cytotoxicity,the MHC Ia expression and IL 1 and TNF secretion of the macrophage were all augmented after i.p injection of IL 2 or IL 6 DNA liposomes, and more efficient activation was observed in the mice treated with IL 2 DNA liposome.IL 2 combined with IL 6 gene therapy showed the maximal activation of macrophages from the lymphoma bearing mice.Conclusion:IL 2 and IL 6 gene therapy can relieve the suppression of macrophages of the lymphoma bearing mice,and activate the antitumor immune responses efficiently.

关 键 词：白细胞介素 巨噬细胞 淋巴瘤 脂质体 

分 类 号：R733.405[医药卫生—肿瘤]