大鼠体外循环肺损伤动物模型的建立  

Establishment of a Rat Model of CPB

作　　者：董啸[1] 何雄[1] 徐建军[1] 

机构地区：[1]南昌大学第二附属医院心胸外科,南昌330006

出　　处：《江西医学院学报》2007年第4期1-4,共4页Acta Academiae Medicinae Jiangxi

基　　金：国家高技术研究发展计划(863计划)(2001AA216061)

摘　　要：目的为研究大鼠体外循环(CPB)中肺损伤机制与肺保护方法,建立大鼠体外循环肺损伤动物模型。方法雄性SD大鼠30只分为两组,麻醉开胸组10只,体外循环组20只(实验鼠和供血鼠各加10只),颈静脉和股动脉插管,建立体外循环,流量120～150 mL/min,预充液10 mL全血,5 mL代血浆,同时胸骨正中切口阻断肺动脉1 h后开放,逐渐脱离体外循环,开胸对照组10只是开胸全麻。结果所有数据均显示10例体外循环动物模型均建立成功。结论大鼠体外循环肺损伤动物模型方法上是可行的,是研究体外循环肺损伤的优良动物模型。Objective To establish a rat model of CPB and to investigate the CPB-related lung dysfanction and possible protective interventions. Methods Male SD rats（240-260 g）were randomly divided into CPB（n=10）group and Sham group（n=10）. All rats were anaesthetized and mechanically ventilated. The femoral artery and vein were cannulated for continuous blood pressure recordings and fluid replacement, respectively. The CPB circuit comprised a venous reservoir,a membrane oxygenator,and a roller pump. Blood was drained from the right atrium via a jugular vein catheter and returned to the right carotid artery. Priming consisted of 10 mL of homologous blood and 5 mL of colloid. CPB was conducted for 90 rain at a flow rate of 1204 150 mL/kg/min in the CPB group. Haemodynamic investigations, blood gas analysis, and survival studies were performed subsequently. Results Our data showed that the rat model principally simulated the clinical setting of CPB in terms of its construction, configuration, performance, material surface area, and priming volume to blood volume ratio. All CPB rats survived and haemodynamic investigations,blood gas analysis were normal. Conclusion The rat model of CPB was easy to establish and should facilitate the investigation of the pathophysiological processes concerning CPB-related lung dysfunction and possible protective interventions.

关 键 词：体外循环 大鼠 肺损伤 

分 类 号：R-332[医药卫生]