视神经脊髓炎的CD4^+和CD8^+T细胞内细胞因子的分析  

Analysis of Intracellular Cytokines of CD4^+ and CD8^+T Cells in Neuromyelitis Optica

作　　者：王卫真[1] 项正兵[1] 吴晓牧[2] 谢旭芳[2] 曹文锋[2] 黄刚[2] 

机构地区：[1]江西省人民医院临床医学研究所 [2]江西省人民医院神经内科,南昌330006

出　　处：《实用临床医学（江西）》2007年第7期8-10,13,共4页Practical Clinical Medicine

摘　　要：目的:探讨视神经脊髓炎(NMO)的免疫反应类型以及在疾病的复发期和缓解期其免疫反应类型的变化情况。方法:利用流式细胞仪并采用细胞内抗原染色的方法对22名临床诊断为NMO的患者(复发期患者8名;缓解期患者14名)进行CD4+和CD8+T细胞内细胞因子白介素(IL)-13I、L-5测定并加以分析。20名健康人为对照组。结果:与健康对照组比较,NMO组的CD4+IL-13+T细胞和CD8+IL-13+T细胞显著增加(P<0.05),但将复发期和缓解期分开来分析时发现仅仅在复发期显著增加,在缓解期无明显变化(P>0.05)。CD4+IL-5+T细胞和CD8+IL-5+T细胞在复发期和缓解期均无显著变化(P>0.05)。结论:NMO的发病及发病阶段与II型辅助性T细胞(Th2)和II型细胞毒性T细胞(Tc2)所分泌的IL-13有关,而与IL-5无关。同时也说明对T细胞内细胞因子进行测定在分析神经系统自身免疫性疾病的发病过程有一定的意义。Objective：To investigate the types of immune response at relapse and remission phases of neuromyelitis optica（NMO） through analysis of the intracellular cytokines.Methods： The intracellular production of interleukin（IL）-5 and IL-13 in peripheral blood CD4＋ and CD8＋ T cells were examined by flow cytometry from patients with NMO.Healthy subjects were also examined as controls.Results：Patients with NMO during relapse phases showed a significantly higher percentage of CD4＋IL-13＋ and CD8＋IL-13＋cells than that in the healthy.However,CD4＋IL-5＋ and CD8＋IL-5＋ cells did not vary regardless of clinical phase or type.Conclusion：Certain kinds of Th2 and Tc2 cytokines may be associated with both the onset and the clinical phase of NMO.The relapse of NMO is related to IL-13,but not IL-5,secreted by Th2 and Tc2.

关 键 词：视神经脊髓炎 T细胞 细胞内细胞因子 

分 类 号：R744.52[医药卫生—神经病学与精神病学]