军曹鱼淋巴囊肿病毒主衣壳蛋白基因全序列分析  

Inverse PCR Amplification of the Complete Major Capsid Protein Gene of Lymphocystis Disease Virus Isolated From Rachycentron canadum and the Phylogenetic Analysis of the Virus

作　　者：付小哲[1] 石存斌[1] 李宁求[1] 潘厚军[1] 常藕琴[1] 吴淑勤[1] 

机构地区：[1]中国水产科学研究院珠江水产研究所广东省水产动物免疫技术重点实验室,广州510380

出　　处：《病毒学报》2007年第5期412-416,共5页Chinese Journal of Virology

基　　金：农业结构调整资助项目(06-05-02B);863计划资助项目(2006AA100308)

摘　　要：The major capsid protein of lymphocystis disease virus isolated from Rachycentron canadum(LCDV-rc) was amplified and analysed.The 457bp DNA core fragment was amplified with the degenerate primers designed according to the conserved sequences of MCP gene of iridoviruses,then the flaking sequences adjacent to the core region were amplified by inverse PCR,and the complete sequence was obtained by combining all of them.The open reading frame of the gene is 1380bp in length,encoding a putative protein of 459 aa with molecular weight 51.12 kD and pI 6.87.Constructing the phylogenetic tree for comparing the MCP amino acid of iridoviruses,the results indicated that LCDV-rc is most homologous to the other Lymphocystis viruses and all of them constitute a branch.Accordingly LCDV-rc is identified as Lymphocystivirus.The major capsid protein of lymphocystis disease virus isolated from Rachycentron canadurn （LCDV-rc） was amplified and analysed. The 457bp DNA core fragment was amplified with the degenerate primers designed according to the conserved sequences of MCP gene of iridoviruses, then the flaking sequences adjacent to the core region were amplified by inverse PCR, and the complete sequence was obtained by combining all of them. The open reading frame of the gene is 1380bp in length, encoding a putative protein of 459 aa with molecular weight 51.12 kD and pI 6.87. Constructing the phylogenetic tree for compa ring the MCP amino acid of iridoviruses, the results indicated that LCDV-rc is most homologous to the other Lymphocystis viruses and all of them constitute a branch. Accordingly LCDV-rc is identified as Lymphocystivirus.

关 键 词：军曹鱼 淋巴囊肿病毒 虹彩病毒 反向PCR 系统进化 

分 类 号：S941.41[农业科学—水产养殖]